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Latest recommendations
Id | Title * | Authors * | Abstract * | Picture | Thematic fields * | Recommender | Reviewers | Submission date▲ | |
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09 Sep 2024
STAGE 1
How Effortful is Boredom? Studying Self-Control Demands Through PupillometryVanessa C. Radtke, Wanja Wolff, Corinna S. Martarelli https://osf.io/eujpa?view_only=12191f02a5db4689b00b42bab7dbd522Unmasking the effort of boredom: A deeper look into self-control dynamicsRecommended by Thomas Meyer based on reviews by Jonas Dora, Julia Englert, Erik Bijleveld and 1 anonymous reviewerEffective effort regulation is central for self-control and has been a focus of psychological research for many decades. It is well established that challenging cognitive tasks, which impose self-control demands due to their complexity, are perceived as more effortful and require greater physiological activation than simpler tasks. However, the experience and allocation of effort during a task, whether easy or difficult, likely depends on more than these inherent self-control demands. Another key factor is boredom, which can arise during task execution. Yet, the role of boredom and the interplay with perceived task difficulty over time remains underexamined.
Based on these considerations, Radtke et al. (2024) aim to examine how boredom and task difficulty influence both subjective and objective measures of effort. They propose that subjective cognitive effort consists of both boredom-related and difficulty-related components, hypothesizing that both factors together more accurately predict objective effort allocation than task difficulty alone. The proposed study will use a within-subject design, in which participants perform two versions of the Stroop task: an easy version with only congruent trials and a hard version. Thought probes during the task will track subjective boredom, fatigue, perceived difficulty, and effort. Phasic and stimulus-evoked pupil dilation will serve as an objective index of cognitive effort, while a subsequent flanker task will assess effects on secondary task performance.
The authors predict that the easy Stroop version will result in lower perceived difficulty but higher boredom, as well as fewer errors and faster reaction times compared to the hard version. Over time, boredom and perceived effort due to boredom are expected to increase, while perceived task difficulty and effort attributed to difficulty are anticipated to decrease. Linear Mixed Models will be employed to determine whether phasic and stimulus-evoked pupil dilation can be predicted by effort attributed to boredom, difficulty, or both. This innovative approach combines objective and subjective measures and promises to deepen our understanding of the cognitive demands posed by boredom, with significant implications for research on effort regulation and motivation.
The Stage 1 manuscript was evaluated by four expert reviewers in two rounds of in-depth review. Following responses from the authors, the recommender determined that Stage 1 criteria were met and awarded in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/38qz4
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly Journals:
References Radtke, V., Wolff, W., & Martarelli, C. S. (2024). How effortful is boredom? Studying self-control demands through pupillometry. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/38qz4
| How Effortful is Boredom? Studying Self-Control Demands Through Pupillometry | Vanessa C. Radtke, Wanja Wolff, Corinna S. Martarelli | <p>Self-control is essential for managing our actions, yet its exertion is perceived as effortful. Performing a task may require effort not only because of its inherent difficulty but also due to its potential for inducing boredom, as boredom has ... | Humanities, Social sciences | Thomas Meyer | 2023-11-21 17:56:13 | View | ||
Genetically-modified animals as models of neurodevelopmental conditions: a review of systematic review reporting qualityEmma Wilson, Gillian Currie, Malcolm Macleod, Peter Kind, and Emily S Sena https://doi.org/10.31222/osf.io/s5xd4Evidence for mixed quality of systematic reviews in preclinical animal studies of neurodevelopmental conditionsRecommended by Chris Chambers based on reviews by Marietta Papadatou-PastouSingle gene alterations have been estimated to account for nearly half of neurodevelopmental conditions (NDCs), providing a crucial opportunity for animal models to understand the underlying mechanisms, causes and potential treatments. The use of systematic reviews (SRs) can, in principle, provide a powerful means to synthesise this evidence-base; however, the reporting quality of previous SRs in preclinical animal research has been found lacking (Hunniford et al., 2021). In the current study, Wilson et al. (2023) will undertook a review of systematic reviews to assess the characteristics and reporting quality of SRs that, in turn, synthesise research in genetically-modified animals to model NDCs. In particular, the authors extracted key features of reviews (including, among others, the aim and primary research questions, relevant animal model, and number of studies in the SR), in addition to quality indicators such as risk of bias and completeness of reporting. In doing so, the authors aimed to enhance guidance on the conduct and reporting of systematic reviews in this area.
Of twelve publications that met the preregistered search criteria, the completeness and quality of reporting was variable. Among the better reported characteristics were search strategies (9 of 12 articles), reporting of funding sources (10 of 12 articles) and use of animal data (11 of 12 articles). In contrast, only two articles reported whether the study protocol was preregistered, only three articles reported methods for assessing risk of bias, and just one included methods to analyse publication bias. In addition, the authors identified 19 review registrations via PROSPERO, most of which remained unpublished after their anticipated end dates. Overall, the results highlight the importance of adherence to reporting guidelines for increasing the transparency and reproducibility of SRs in this field.
The Stage 2 manuscript was evaluated over one round of in-depth review. Based on detailed responses by the authors, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation.
URL to the preregistered Stage 1 protocol: https://osf.io/952qk Level of bias control achieved: Level 4. At least some of the data/evidence that was used to answer the research question already existed prior to IPA and was accessible in principle to the authors, but the authors certify that they did not access any part of that data/evidence prior to IPA.
List of eligible PCI RR-friendly journals: References
1. Hunniford V. T., Montroy J., Fergusson D. A., Avey M. T., Wever K. E., McCann S. K., Foster M., Fox G., Lafreniere M., Ghaly M., Mannell S., Godwinska K., Gentles A., Selim S., MacNeil J., Sikora L., Sena E. S., Page M. J., Macleod M., Moher D., & Lalu M. M. (2021). Epidemiology and reporting characteristics of preclinical systematic reviews. PLOS Biology, 19:e3001177. https://doi.org/10.1371/journal.pbio.3001177
2. Wilson, E., Currie, G., Macleod, M., Kind, P. & Sena, E. S. (2023). Genetically-modified animals as models of neurodevelopmental conditions: a review of systematic review reporting quality [Stage 2]. Acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/s5xd4
| Genetically-modified animals as models of neurodevelopmental conditions: a review of systematic review reporting quality | Emma Wilson, Gillian Currie, Malcolm Macleod, Peter Kind, and Emily S Sena | <p><strong>Objective</strong><br>Using genetically-modified animals to model neurodevelopmental conditions (NDCs) helps better our understanding of biology underlying these conditions. Animal research has unique characteristics not shared with cli... | Medical Sciences | Chris Chambers | 2023-11-22 10:26:44 | View | ||
21 Apr 2024
STAGE 1
Mechanisms of secularization: Testing between three causal pathwaysMartin Lang, Radim Chvaja https://osf.io/scnuh?view_only=2afb8c616e6245debd279ee3f0b19675Understanding links between secularization, rationalisation and insecurityRecommended by Adrien Fillon based on reviews by Chris Chambers and 1 anonymous reviewerWhat relationship can be expected between secularization, rationalisation and insecurity? While some authors argue that rationalisation reduces the willingness to belong to religious groups, others have suggested that insecurity increases this need to belong to religious groups.
In the current study, Lang and Chvaja (2024) will adjudicate between these two possibilities using an economics game in participants from two countries: US and Poland. The central question posed by the authors is whether cooperative insecurity increases the probability of joining a religious normative group. They will test the relationship between an environment (secure and insecure) and institution (which related to the norm context: religious and secular) on the probability of choosing the normative group in an experimental setting. Therefore, the study will be a quantitative analysis.
The authors included an adequate power analysis, alternatives for non-supported hypotheses, and filtering to ensure a high quality of data collection. They also undertook a pilot study to ensure the quality of the procedure and sensitivity of the analyses.
The Stage 1 manuscript was evaluated over one round of in-depth review. Based on detailed responses to reviewers’ and the recommender’s comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance.
URL to the preregistered Stage 1 protocol: https://osf.io/yzgek
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
List of eligible PCI RR-friendly journals:
References 1.Lang, M. & Chvaja, R. (2024). Mechanisms of secularization: Testing between the rationalization and existential insecurity theories. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/yzgek
| Mechanisms of secularization: Testing between three causal pathways | Martin Lang, Radim Chvaja | <p>The study tests two competing explanations of the secularization process related to rationalizing worldviews and decreasing existential insecurity. While the former explanation argues that people are unwilling to join religious groups because o... | Social sciences | Adrien Fillon | 2023-11-22 11:17:30 | View | ||
25 Jun 2024
STAGE 1
Does ‘virtuality’ affect the role of prior expectations in perception and action? Comparing predictive grip and lifting forces in real and virtual environmentsDavid J. Harris, Tom Arthur, & Gavin Buckingham https://osf.io/q3ktsThe role of prior expectations for lifting objects in virtual realityRecommended by Robert McIntosh based on reviews by 2 anonymous reviewersAs virtual reality environments become more common, it is important to understand our sensorimotor interactions with them. In real world settings, sensory information is supplemented by prior expectations from past experiences, aiding efficient action control. In VR, the relative role of expectations could decrease due to a lack of prior experience with the environment, or increase because sensory information is impoverished or ambiguous. Harris, Arthur and Buckingham (2024) propose to test these possibilities by comparing a real-world object lifting task and a VR version in which the same objects are lifted but visual feedback is substituted by a virtual view. The experiment uses the Size-Weight Illusion (SWI) and the Material Weight Illusion (MWI). In these paradigms, the visual appearance of the object induces expectations about weight that can affect the perception of weight during lifting, and the fingertip forces generated. The degree to which the visual appearance of objects induces differences in perceived weight, and in measured fingertip forces, will index the influence of prior expectations for these two paradigms. The analyses will test whether the influence of prior expectations is lower or higher in the VR set-up than in real-world lifting. The outcomes across tasks (SWI and MWI) and measures (perceived weight, fingertip forces) will broaden our understanding of the role of predictive sensorimotor control in novel virtual environments.
After three rounds of evaluation, with input from two external reviewers, the recommender judged that the Stage 1 manuscript met the criteria for in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/36jhb
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly journals:
References 1. Harris, D. J., Arthur, T., & Buckingham, G. (2024). Does ‘virtuality’ affect the role of prior expectations in perception and action? Comparing predictive grip and lifting forces in real and virtual environments. In principle acceptance of Version 4 by Peer Community in Registered Reports. https://osf.io/36jhb
| Does ‘virtuality’ affect the role of prior expectations in perception and action? Comparing predictive grip and lifting forces in real and virtual environments | David J. Harris, Tom Arthur, & Gavin Buckingham | <p>Recent theories in cognitive science propose that prior expectations strongly influence how individuals perceive the world and control their actions. This influence is particularly relevant in novel sensory environments, such as virtual reality... | Life Sciences | Robert McIntosh | Ben van Buren | 2023-11-22 12:25:57 | View | |
27 Mar 2024
STAGE 1
Revisiting the signal value of emotion in altruistic behavior: Replication and extensions Registered Report of Barasch et al. (2014) Studies 3 and 6Tse Lyn (Rachael) Woo; Gilad Feldman https://osf.io/7ym9nUnderstanding how motives and emotions driving prosocial actions impact the moral assessment of good doersRecommended by Romain Espinosa based on reviews by Thibaut Arpinon and Angela SutanPro-social actions are often driven by emotional factors. For instance, emotions have been shown to play a decisive role in the way we judge the fairness of a situation (affect-as-information theory: e.g., Clore et al., 2001; Storbeck and Clore, 2008), and, thus, how we make decisions. Specific emotions like anger have also been shown to stimulate the motivation to fight injustice (Lerner et al., 2015). At the individual level, people might undertake altruistic actions as a way to relieve themselves from these negative emotions (what Cialdini (1991) calls ‘reflexive distress’) but also because these actions are expected by the social norms (‘normative distress’). Indeed, pro-social actions are usually taken in social contexts, and the perception of one’s behavior by third parties might hinder or facilitate the adoption of pro-social behaviors. Understanding the determinants of the perception of altruistic behaviors is thus a key research question to support pro-social actions in collective settings.
In the current study, Woo and Feldman (2024) aim to replicate the seminal work of Barasch et al. (2014), who showed that third parties hold more favorable views of agents undertaking pro-social actions when the latter are motivated by emotions. More precisely, the authors aim to replicate two studies of the original work by conducting a well-powered online experiment (US participants, Prolific, N=1,164). First, they will investigate whether donors who exhibit higher distress regarding the suffering of others are perceived as more moral and authentically concerned for others. Second, they will analyze whether individuals who expect material or reputational benefits from their altruistic deeds are perceived by third parties as less moral than those who act for emotional reasons. In addition to these two replication objectives, the authors propose extensions with pre-registered hypotheses that are inspired by Study 2 from the original work. They seek to investigate whether people are seen as more other-focused when they undertake a prosocial action (donation) and under different expected rewards (material, reputational, emotional benefits).
The Stage 1 manuscript was evaluated by two external reviewers and the recommender. Based on detailed responses to the reviewers' and the recommender’s comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/d5bmp
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly journals:
References 1. Barasch, A., Levine, E. E., Berman, J. Z., & Small, D. A. (2014). Selfish or selfless? On the signal value of emotion in altruistic behavior. Journal of Personality and Social Psychology, 107, 393-413. https://doi.org/10.1037/a0037207 2. Cialdini, R. B. (1991). Altruism or egoism? That is (still) the question. Psychological Inquiry, 2, 124-126. https://psycnet.apa.org/doi/10.1207/s15327965pli0202_3
3. Clore, G. L., Gasper, K., Garvin, E., & Forgas, J. P. (2001). Handbook of Affect and Social Cognition.
4. Lerner, J. S., Li, Y., Valdesolo, P., & Kassam, K. S. (2015). Emotion and decision making. Annual Review of Psychology, 66, 799-823. https://doi.org/10.1146/annurev-psych-010213-115043
5. Storbeck, J., & Clore, G. L. (2008). Affective arousal as information: How affective arousal influences judgments, learning, and memory. Social and Personality Psychology Compass, 2, 1824-1843. https://doi.org/10.1111%2Fj.1751-9004.2008.00138.x
6. Woo, T. L. & Feldman, G. (2024). Revisiting the signal value of emotion in altruistic behavior: Replication and extensions Registered Report of Barasch et al. (2014) Studies 3 and 6. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/d5bmp
| Revisiting the signal value of emotion in altruistic behavior: Replication and extensions Registered Report of Barasch et al. (2014) Studies 3 and 6 | Tse Lyn (Rachael) Woo; Gilad Feldman | <p>[IMPORTANT: Abstract, method, and results were written using a randomized dataset produced by Qualtrics to simulate what these sections will look like after data collection. These will be updated following the data collection. For the purpose o... | Social sciences | Romain Espinosa | 2023-11-23 05:22:23 | View | ||
30 May 2024
STAGE 1
Gaming Disorder: A Qualitative Meta-synthesis of Case StudiesTiina Auranen, Matúš Adamkovič, Veli-Matti Karhulahti, Marcel Martoncik, Yaewon Jin, Miia Siutila, Valtteri Kauraoja, Solip Park https://osf.io/sv2rmWhat can qualitative research tell us about Gaming Disorder?Recommended by Mateo Leganes-Fonteneau and Chris Chambers based on reviews by Simone Amendola, Gemma Lucy Smart and Ting PanHow can qualitative research and case studies inform theoretical models of gaming disorder? Gaming Disorder has generated a large amount of research, with up to 95% of it focusing on quantitative studies. As a result, most of the meta-analyses and review studies focus on quantitative research and disregard qualitative approaches. However, studying the direct experiences of gamers can provide more detailed and direct evidence that can feed into theoretical models.
In this Stage 1 manuscript, Auranen et al. (2024) will perform a qualitative meta-analysis of gaming disorder case studies, case reports, and case series of treatment-seekers using a 3-phase approach. First, they will focus on examining the reported problems of participants, the reported reasons for seeking help to reduce their gaming, and the causal link between gaming and reported problems, including the context of these problematic behaviors. Second, thematic synthesis will generate construct themes regarding the contexts of the individuals and the reported problems. Lastly, the authors will examine the relevance of findings in regards to the coping model, value fulfillment theory and the theory of cultural dissonance.
The Stage 1 submission was evaluated by the recommender and three expert reviewers, one of which was recused due to conflicts of interest that emerged after the first round of revisions. The recommender and co-recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/hzc5a
Level of bias control achieved: Level 4. At least some of the data/evidence that will be used to answer the research question already exists AND is accessible in principle to the authors (e.g. residing in a public database or with a colleague), BUT the authors certify that they have not yet accessed any part of that data/evidence.
List of eligible PCI RR-friendly journals: References Auranen, T., Adamkovič, M., Martončik, M., Park, S., Kauraoja, V., Siutila, M., Jin, Y., & Karhulahti, V.-M. (2024). Gaming Disorder: A qualitative meta-synthesis of related case studies. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/hzc5a
| Gaming Disorder: A Qualitative Meta-synthesis of Case Studies | Tiina Auranen, Matúš Adamkovič, Veli-Matti Karhulahti, Marcel Martoncik, Yaewon Jin, Miia Siutila, Valtteri Kauraoja, Solip Park | <p>Several meta-analyses and reviews have been published on gaming-related health problems, which are today studied mainly in the context of the World Health Organization’s new mental disorder construct, “gaming disorder”. However, none of those r... | Medical Sciences, Social sciences | Mateo Leganes-Fonteneau | Ting Pan | 2023-11-24 14:00:19 | View | |
01 Jun 2024
STAGE 1
Can Imagining Actions as Occurring Involuntarily Cause Intentional Behaviour to Feel Involuntary?Kevin Sheldrake, Zoltan Dienes https://osf.io/74gcnCan the sense of agency and reality be altered by our meta-cognitive models?Recommended by Anoushiravan Zahedi based on reviews by Zoltan Kekecs and Sophie SiestrupAlterations in subjective experience, including alterations in the sense of agency (SoA) and reality (SoR), are commonly implicated in direct-verbal suggestions, such as hypnotic suggestions. Although extensively studied, how direct-verbal suggestions can alter the SoA and SoR is not understood (e.g., see Martin & Pacherie, 2019; Zahedi et al., 2024). One class of theories postulates that the alterations in SoA and SoR are related to meta-cognition. For instance, the intention to move or form a mental image can be kept out of conscious awareness, creating a sense of involuntariness (Dienes & Perner, 2007).
Relying on this theory, in the current study Sheldrake and Dienes (2024) postulate that the metacognitive processes related to these alterations can occur by appropriate use of imagination. In other words, by imagining the movement or object to be hallucinated and further imagining the underlying process was outside of awareness, one can elicit alterations in SoA and SoR. To this end, an intervention is devised whereby the participant is repeatedly asked to consider what might help or hinder them from imagining they are unaware of the relevant intention and thereby adjust their imagination. A control group will be asked to increase the feeling of involuntariness or altered reality simply by repeated practice. Afterward, participants will be asked in a test phase the extent to which the suggested experience felt involuntary. The Stage 1 manuscript was evaluated over three rounds of in-depth review. Based on detailed responses to the reviewers' comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/f8hsd Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly journals: References 1. Dienes, Z. & Perner, J. (2007). Executive control without conscious awareness: The cold control theory of hypnosis. In G. A. Jamieson (Ed.), Hypnosis and conscious states: The cognitive neuroscience perspective (pp. 293-314). Oxford University Press.
2. Martin, J. R. & Pacherie, E. (2019). Alterations of agency in hypnosis: A new predictive coding model. Psychol Rev, 126(1), 133-152. https://doi.org/10.1037/rev0000134
3. Sheldrake, K. & Dienes, Z. (2043). Can Imagining Actions as Occurring Involuntarily Cause Intentional Behaviour to Feel Involuntary? In principle acceptance of Version 6 by Peer Community in Registered Reports. https://osf.io/f8hsd
4. Zahedi, A., Lynn, S. J., & Sommer, W. (2024). Cognitive Simulation along with Neural Adaptation Explain Effects of Suggestions: A Novel Theoretical Framework. Frontiers in Psychology. https://doi.org/10.3389/fpsyg.2024.1388347
| Can Imagining Actions as Occurring Involuntarily Cause Intentional Behaviour to Feel Involuntary? | Kevin Sheldrake, Zoltan Dienes | <p>The cold control theory of response to imaginative suggestions calling for distortions in veridical experience (including hypnotic suggestions) states that behavioural and cognitive responses are generated intentionally, but are perceived as in... | Social sciences | Anoushiravan Zahedi | Zoltan Kekecs | 2023-11-25 16:24:53 | View | |
31 May 2024
STAGE 1
Representativeness heuristic in intuitive predictions: Replication Registered Report of problems reviewed in Kahneman and Tversky (1973)Hong Ching (Bruce) Chan, Gilad Feldman https://osf.io/9cqp6The Representativeness Heuristic Revisited: Registered Replication Report of Kahneman and Tversky (1973)Recommended by Rima-Maria Rahal based on reviews by Peter Anthony White, Regis Kakinohana and Naseem Dillman-HassoRevisiting a true classic, this registered replication report addresses Kahneman and Tversky’s (1973) introduction of the representativeness heuristic. The heuristic refers to deviations of judgments from normative evaluations of the evidence when the stimulus fits to a prototype. For instance, when an individual is described by features stereotypically associated with a certain target group (e.g., a person who attends dance training several times a week and has a passion for singing and performing), likelihood judgments that the individual belongs to a target group (K-Pop artists) compared to a non-target group (e.g., accountants) are inflated.
The impact of the original research on the field is clearly immense and long-lasting. All the better that a systematic replication attempt is being undertaken in this registered report, which addresses studies 1 through 7 of Kahneman and Tversky’s classic 1973 paper. Chan and Feldman (2024) propose a well-powered online study, in which all studies from the original article are presented to participants within-subjects. The materials are carefully constructed and closely documented in the accompanying OSF project, where in-depth information on planned data analyses is supported with a simulated dataset. The Stage 1 manuscript was evaluated over three rounds of in-depth review. Based on detailed responses to the reviewers' comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA). URL to the preregistered Stage 1 protocol: https://osf.io/er2cq
Level of bias control achieved: Level 6. Data collection commenced during the later part of Stage 1 peer review; however, since no substantive changes to the design were made after this point, the risk of bias due to prior data observation remains zero and the manuscript therefore qualifies for Level 6.
List of eligible PCI RR-friendly journals:
References
1. Chan, H. C. & Feldman, G. (2024). Representativeness heuristic in intuitive predictions: Replication Registered Report of problems reviewed in Kahneman and Tversky (1973). In principle acceptance of Version 5 by Peer Community in Registered Reports. https://osf.io/er2cq 2. Kahneman, D., & Tversky, A. (1973). On the psychology of prediction. Psychological Review, 80(4), 237–251. https://doi.org/10.1037/h0034747
| Representativeness heuristic in intuitive predictions: Replication Registered Report of problems reviewed in Kahneman and Tversky (1973) | Hong Ching (Bruce) Chan, Gilad Feldman | <p>[IMPORTANT: Abstract, method, and results were written using a randomized dataset produced by Qualtrics to simulate what these sections will look like after data collection. These will be updated following the data collection. For the purpose o... | Social sciences | Rima-Maria Rahal | 2023-11-29 15:19:07 | View | ||
22 Jul 2024
STAGE 1
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”Maha Said, Mustafa Gharib, Samia Zrig, Raphaël Lévy https://osf.io/kf9qe/Replicating, Revising and Reforming: Unpicking the Apparent Nanoparticle Endosomal Escape ParadoxRecommended by Emily Linnane and Yuki Yamada based on reviews by Cecilia Menard-Moyon and Zeljka KrpeticContext
Over the past decade there has been an exponential increase in the number of research papers utlising nanoparticles for biological applications such as intracellular sensing [1, 2], theranostics [3-5] and more recently drug delivery and precision medicine [6, 7]. Despite the success stories, there is a disconnect regarding current dogma on issues such as nanoparticle uptake and trafficking, nanoparticle delivery via the enhanced permeability and retention (EPR) effect, and endosomal escape. Critical re-evaluation of these concepts both conceptually and experimentally is needed for continued advancement in the field.
For this preregistration, Said et al. (2024) focus on nanoparticle intracellular trafficking, specifically endosomal escape [8]. The current consensus in the literature is that nanoparticles enter cells via endocytosis [9, 10] but reportedly just 1-2% of nanoparticles/ nanoparticle probes escape endosomes and enter the cytoplasm [11-13]. There is therefore an apparent paradox over how sensing nanoparticles can detect their intended targets in the cytoplasm if they are trapped within the cell endosomes. To address this fundamental issue of nanoparticle endosomal escape, Lévy and coworkers are carrying out replication studies to thoroughly and transparently replicate the most influential papers in the field of nanoparticle sensing. The aim of these replication studies is twofold: to establish a robust methodology to study endosomal escape of nanoparticles, and to encourage discussions, transparency and a step-change in the field.
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”
For this replication study, the authors classified papers on the topic of nanoparticle sensing and subsequently ranked them by number of citations. Based on this evaluation they selected a paper by Zhu and colleagues [14] entitled “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions” for their seminal replication study. To determine the reproducibility of the results from Zhu et al., the authors aim to establish the proportion of endosomal escape of the nanoparticles, and to examine the data in a biological context relevant to the application of the probe.
Beyond Replication
The authors plan to replicate the exact conditions reported in the materials and methods section of the selected paper such as nanoparticle probe synthesis of CdSe@C-TPEA nanoparticles, assessment of particle size, stability and reactivity and effect on cells (TEM, pH experiments, fluorescent responsivity to metal ions and cell viability). In addition, Said et al., plan to include further experimental characterisation to complement the existing study by Zhu and colleagues. They will incorporate additional controls and methodology to determine the intracellular location of nanoparticle probes in cells including: quantifying excess fluorescence in the culture medium, live cell imaging analysis, immunofluorescence with endosomal and lysosomal markers, and electron microscopy of cell sections. The authors will also include supplementary viability studies to assess the impact of the nanoparticles on HeLa cells as well as an additional biologically relevant cell line (for use in conjunction with the HeLa cells as per the original paper).
The Stage 1 manuscript underwent two rounds of thorough in-depth review. After considering the detailed responses to the reviewers' comments, the recommenders determined that the manuscript met the Stage 1 criteria and awarded in-principle acceptance (IPA).
The authors have thoughtfully considered their experimental approach to the replication study, whilst acknowledging any potential limitations. Given that conducting such a replication study is novel in the field of Nanotechnology and there is currently no ‘gold standard’ approach in doing so, the authors have showed thoughtful regard of statistical analysis and unbiased methodology where possible.
Based on current information, this study is the first use of preregistration via Peer Community in Registered Reports and the first formalised replication study in Nanotechnology for Biosciences. The outcomes of this of this study will be significant both scientifically and in the wider context in discussion of the scientific method.
URL to the preregistered Stage 1 protocol: https://osf.io/qbxpf
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly Journals:
References
1. Howes, P. D., Chandrawati, R., & Stevens, M. M. (2014). Colloidal nanoparticles as advanced biological sensors. Science, 346(6205), 1247390. https://doi.org/10.1126/science.1247390
2. Liu, C. G., Han, Y. H., Kankala, R. K., Wang, S. B., & Chen, A. Z. (2020). Subcellular performance of nanoparticles in cancer therapy. International Journal of Nanomedicine, 675-704. https://doi.org/10.2147/IJN.S226186
3. Tang, W., Fan, W., Lau, J., Deng, L., Shen, Z., & Chen, X. (2019). Emerging blood–brain-barrier-crossing nanotechnology for brain cancer theranostics. Chemical Society Reviews, 48(11), 2967-3014. https://doi.org/10.1039/C8CS00805A
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| Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions” | Maha Said, Mustafa Gharib, Samia Zrig, Raphaël Lévy | <p>In hundreds of articles published over the past two decades, nanoparticles have been described as probes for sensing and imaging of a variety of intracellular cytosolic targets. However, nanoparticles generally enter cells by endocytosis with o... | Life Sciences, Medical Sciences, Physical Sciences | Emily Linnane | 2023-11-29 19:14:03 | View | ||
19 Jun 2024
STAGE 1
Culture-Driven Neural Plasticity and Imprints of Body-Movement Pace on Musical Rhythm ProcessingSégolène M. R. Guérin, Emmanuel Coulon, Tomas Lenc, Rainer Polak, Peter E. Keller, Sylvie Nozaradan https://zenodo.org/doi/10.5281/zenodo.10221480The interplay of music, movement, and culture on rhythm processingRecommended by Juan David Leongómez based on reviews by Anne Keitel and 1 anonymous reviewerThe interplay of music, movement, and cultural experience shapes rhythm perception. From bouncing babies and children at play, to tapping, clapping, and dancing, music often triggers synchronous body movements that can influence how we process rhythm. And, at the same time, long-term exposure to specific musical traditions shapes how we perceive and interpret rhythms.
However, direct behavioural and neuroscientific evidence on how these processes occur remains scarce. In this programmatic submission, comprising two complementary Stage 2 reports, Guérin et al. (2024) will investigate how body movements shape the processing of auditory information, and how previous short-term motor practice and long-term cultural experience interact to shape neural and behavioural responses to rhythmic stimuli. The authors will record in separate sessions both electroencephalography (EEG) and hand clapping, in response to rhythms from West/Central Africa. These recordings will be conducted before and after a session in which participants will clap/step to either a three or a four-beat metre that is expected to influence how they interpret a rhythm.
The first Stage 2 report, which will be conducted on African-enculturated participants, aims to demonstrate how body-movement pace flexibly imprints on human sensory processing. The authors build on various theoretical models that emphasise the role of motor production in metre perception, and predict that both neural and behavioural entrainment will improve following movement, matching the rhythmic pattern set by the previous body movements.
The second Stage 2 report aims to uncover how short-term motor practice and long-term cultural experience interact to shape responses to rhythmic stimuli, by integrating short-term motor practice and long-term cultural experience. For this, the authors will test separate groups of participants from distinct cultural backgrounds (African vs. Western-enculturated), which are predicted to show neural and behavioural differences in their preferred metric mapping before body movement, and expect that neural and behavioural entrainment will improve after movement, especially for the metre set by prior movements, and more significantly for the metre common in the participant's culture.
Together, the findings from the planned Stage 2 reports are expected to clarify how long-term cultural background and short-term motor practice imprint onto rhythm processing in humans. This research will enhance our understanding of how cultural experience, body movement, and neural plasticity interact in music processing.
The Stage 1 manuscript was evaluated over three rounds of in-depth review. Based on detailed responses to the reviewers' and recommender's comments, the recommender judged that the manuscript met the Stage 1 criteria and awarded in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/skuyc
Level of bias control achieved: Level 6. Data collection commenced during the later part of Stage 1 peer review; however, since no changes to the design were made after this point, the risk of bias due to prior data observation remains zero and the manuscript therefore qualifies for Level 6. List of eligible PCI RR-friendly journals: References
Guérin, S. M. R., Coulon, E., Lenc, T., Polak, R., Keller, P. E., & Nozaradan, S. (2024). Culture-Driven Neural Plasticity and Imprints of Body-Movement Pace on Musical Rhythm Processing. In principle acceptance of Version 2.1 by Peer Community in Registered Reports. https://osf.io/skuyc
| Culture-Driven Neural Plasticity and Imprints of Body-Movement Pace on Musical Rhythm Processing | Ségolène M. R. Guérin, Emmanuel Coulon, Tomas Lenc, Rainer Polak, Peter E. Keller, Sylvie Nozaradan | <p>The proposed programmatic registered report aims at capturing direct neuroscientific evidence for the rhythmic, movement-related shaping of auditory information with a cross-cultural perspective. Specifically, West/Central African- and Western-... | Life Sciences | Juan David Leongómez | Anonymous | 2023-11-30 11:36:06 | View |
MANAGING BOARD
Chris Chambers
Zoltan Dienes
Corina Logan
Benoit Pujol
Maanasa Raghavan
Emily S Sena
Yuki Yamada