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Latest recommendations
| Id | Title * | Authors * | Abstract * | Picture▲ | Thematic fields * | Recommender | Reviewers | Submission date | |
|---|---|---|---|---|---|---|---|---|---|
The effect of covert visual attention on pupil size during perceptual fadingDoes pupil size track high-level attention?Recommended by D. Samuel Schwarzkopf based on reviews by Sander Nieuwenhuis and Martin Rolfs
Higher-level cognitive processes like attention, memory, or mental imagery can produce pupil responses, without any actual difference in luminance of the visual stimulus. Interestingly, the inverse scenario remained untested to date: when a physical luminance difference is perceptually eliminated from awareness, does pupil size still track attention to the stimulus? In this Registered Report, Vilotijević and Mathôt (2024) sought to test this experimentally using a perceptual fading phenomenon where two Gaussian patches with different luminances fade from consciousness and are thus perceived as mid-level uniform grey (or at least the subjective difference is much reduced). This fading manipulation, as well as a control condition without perceptual fading, were presented in separate blocks. Participants were instructed to covertly attend one of the patches.
The authors hypothesised that if pupil size reflects attentional selection, these pupil responses in the fading condition should be eliminated or at least reduced, and this should evolve with time as the stimuli are perceptually fading. Their results show that pupil responses during covert attention are indeed reduced during perceptual fading - but they are not eliminated. Interestingly, this reduction did not depend on time or self-reports of the strength of perceptual fading. The findings therefore suggest that pupil dilation tracks subjective brightness differences. One inherent issue with experiments like these is that the experimental and control conditions necessarily involve a physical difference in the stimulus. Here, the fading condition had the same spatial configuration of light and dark stimuli throughout a block while in the control (non-fading) condition the light and dark stimuli alternated sides between trials. It is therefore impossible to completely rule out that the physical difference affects the results. However, the only alternative to this would be an experimental design in which the stimuli never change, but only the subjective perceptual state varies. Such a design is completely at the mercy of the participant's subjective state and therefore loses experimental control and statistical sensitivity. The present results confirmed the authors' prediction that there are indeed differences in overall pupil responses during the fading and control conditions, irrespective of covert attention.
Critically, the fact that the attention effect did not vary with time or subjective self-reports of the illusion supports the authors' interpretation that this reflects higher-level cognition: the mere act of attending to the dark side - even if the actual appearance has faded - could cause a sustained pupil dilation. This would be consistent with the type of pupil effects for memory and mental imagery that motivated the present study. However, a simpler alternative is that the experience of perceptual fading was incomplete (as possibly suggested by Figures 2D and 2F) but that self-reports fail to capture this subjective experience accurately. Perhaps a future study could compare the magnitude of the attentional pupil effects when the initial stimulus is completely removed. If similar differences in pupil response persist this would suggest that the present results are due to high-level modulation or the residual low-level luminance difference.
The Stage 2 manuscript was evaluated by two reviewers and the recommender over two rounds of review. One reviewer again advised additional robustness checks to rule out eye movement confounds, an issue they had already raised during Stage 1 review. The researchers provide clear evidence that this is unlikely to have confounded their findings. This has been added to the supplementary data repository. Following this review and revision, the recommender judged that the Stage 2 criteria were met and awarded a positive recommendation.
URL to the preregistered Stage 1 protocol: https://osf.io/bmtp6
Level of bias control achieved: Level 6. No part of the data or evidence that was used to answer the research question was generated until after IPA. List of eligible PCI RR-friendly journals:
References
1. Vilotijević, A. & Mathôt, S. (2024). The effect of covert visual attention on pupil size during perceptual fading [Stage 2]. Acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/ku8qc?view_only=f331df53b50f431386fabba9e386b387
| The effect of covert visual attention on pupil size during perceptual fading | Ana Vilotijević, Sebastiaan Mathôt | <p>Pupil size is modulated by various cognitive factors such as attention, working memory, mental imagery, and subjective perception. Previous studies examining cognitive effects on pupil size mainly focused on inducing or enhancing a subjective e... | Social sciences | D. Samuel Schwarzkopf | 2024-08-17 12:58:58 | |||
08 Feb 2022
STAGE 1
Arithmetic deficits in Parkinson's Disease? A registered reportGetting the numbers right in Parkinson's disease?Recommended by Zoltan Dienes based on reviews by Pia Rotshtein, Ann Dowker, Stephanie Rossit and 1 anonymous reviewer
Everyday life, including for patients taking different types of medicine, involves dealing with numbers. Even though Parkinson's disease may ordinarily be thought of as primarily being a motor disorder, there is evidence that numerical abilities decline as Parkinson's disease progresses. Further, the brain areas involved in arithmetic operations overlap with the areas that degenerate in Parkinson's disease. In this Stage 1 Registered Report, Loenneker et al. (2022) will test healthy controls, Parkinson disease patients with normal cognition, and Parkinson disease patients with mild cognitive impairment on general working memory tasks as well as arithmetic performance on the four basic operations (addition, subtraction, multiplication, division). The study aims to test whether or not there is a deficit in each operation, and the relation of any deficits to general working memory capacity. The Stage 1 manuscript was evaluated over four rounds of review (including two rounds of in-depth specialist review). Based on comprehensive responses to the reviewers' comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA). URL to the preregistered Stage 1 protocol: https://osf.io/nb5fj Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly journals:
References Loenneker, H. D., Liepelt-Scarfone, I., Willmes, K., Nuerk, H.-C., & Artemenko, C. (2022). Arithmetic deficits in Parkinson’s Disease? A Registered Report. Stage 1 preregistration, in principle acceptance of version 4 by Peer Community in Registered Reports. https://osf.io/nb5fj | Arithmetic deficits in Parkinson's Disease? A registered report | Hannah D. Loenneker, Inga Liepelt-Scarfone, Klaus Willmes, Hans-Christoph Nuerk, & Christina Artemenko | <p>Elderly people and patients with neurodegenerative diseases such as Parkinson’s Disease (PD) immensely rely on arithmetic skills to lead an independent life. Activities such as medication management, financial transactions or using public trans... | | Life Sciences | Zoltan Dienes | 2021-06-29 19:23:53 | ||
Life Thinning and Gaming Disorder: A Longitudinal Qualitative Registered ReportHow do intensive gaming experiences evolve over time in clinical and non-clinical contexts?Recommended by Chris Chambers based on reviews by Peter Branney and Michelle Carras
Over the last 5 years the inclusion of “gaming disorder” in the ICD-11 been controversial (Van Rooij et al, 2018), mirroring wider public debate about the effects of gaming on mental health. One of the major gaps in understanding the validity of gaming disorder as an identifiable mental illness is the absence of qualitative studies comparing the lived experience of gamers who seek treatment with esports players who do not report health problems.
Here, Karhulahti et al. (2023) tackle this question in the second of two Stage 2 Registered Reports associated with their previous programmatic Stage 1 submission. Using interpretative phenomenological analysis, the authors undertook in-depth interviews over a 1-year period with treatment-seeking participants (N=5) and esports-playing participants (N=4) who did not experience gaming-related health problems. The authors sought to answer the folllwing primary question: How do the experiences and meanings of playing videogames—shaped by the individuals’ diverse sociocultural contexts—evolve in those with related health problems (as defined by treatment-seeking) and those who play esports games several hours per day while self-reporting no related health problems?
Both groups exhibited intense relationships with gaming that were cyclical over time across various dimensions, with fluctuations occurring in response to changes in health, occupation, and social networks. The observed variation over time was substantial, with individuals attaching and detaching from games involving hundreds or thousands of hours. The authors report treatment-seeking being followed by a search of new gaming and life meanings, while intensive gaming without related problems continued as an integrated part of the self, with resilience adapting and evolving in the face of unexpected life events. Taking into account their findings, the authors propose life thinning and resilience integration processes to help describe and explain how some individuals end up seeking treatment for their gaming, while for others gaming supports them and becomes integrated into their identity.
Following one round of in-depth review and revision, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation.
URL to the preregistered Stage 1 protocol: https://osf.io/a2rwg
Level of bias control achieved: Level 4. At least some of the data/evidence that was used to answer the research question existed prior to in-principle acceptance(IPA) but the authors certify that they did not access any part of that data/evidence prior to IPA.
List of eligible PCI RR-friendly journals:
References
1. van Rooij AJ, Ferguson CJ, Carras MC, Kardefelt-Winther D, Shi J, Aarseth E, Bean AM, Bergmark KH, Brus A, Coulson M, Deleuze J, Dullur P, Dunkels E, Edman J, Elson M, Etchells PJ, Fiskaali A, Granic I, Jansz J, Karlsen F, Kaye LK, Kirsh B, Lieberoth A, Markey P, Mills KL, Nielsen RKL, Orben A, Poulsen A, Prause N, Prax P, Quandt T, Schimmenti A, Starcevic V, Stutman G, Turner NE, Looy J van, Przybylski AK (2018) A weak scientific basis for gaming disorder: Let us err on the side of caution. Journal of Behavioral Addictions, 7, 1–9. https://doi.org/10.1556/2006.7.2018.19
2. Karhulahti V-M, Siutila M, Vahlo J, Koskimaa R (2023). Life Thinning and Gaming Disorder: A Longitudinal Qualitative Registered Report [Stage 2 Registered Report], acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/hmcqz
| Life Thinning and Gaming Disorder: A Longitudinal Qualitative Registered Report | Veli-Matti Karhulahti, Miia Siutila, Jukka Vahlo, Raine Koskimaa | <p>The academic debates regarding the psychiatric relevance of gaming disorder continue largely because the lived experiences of treatment-seekers remain mostly unstudied. This registered report addresses the above research gap with a longitudinal... |  | Humanities, Medical Sciences, Social sciences | Chris Chambers | 2023-05-15 11:01:37 | ||
21 Feb 2022
STAGE 1
Recommended by Benoit Pujol based on reviews by Jean-François Gerard, Rachel Harrison and 1 anonymous reviewer
This submission has been withdrawn (see notice below)Sex-biased dispersal is widely acknowledged to influence range expansion and the geographic limits of species (Trochet et al. 2016). Evidence is accruing that suggests an impact of the learning ability of species on their capacity to colonise new habitats because the ability to learn provides an advantage when confronted to novel challenges (Lee and Thornton 2021). Whether these two mechanisms interact to shape range expansion remains however unknown. One could expect this interaction because both dispersal and the ability to learn are linked to related behaviours (e.g., exploration, Lee and Thornton 2021). In their study entitled “Investigating sex differences in learning in a range-expanding bird”, Alexis J. Breen and Dominik Deffner (Breen and Deffner 2022) propose to test this hypothesis in range-expanding great-tailed grackles (Quiscalus mexicanus) by exploring the individual variation of several behavioural traits (e.g., exploration, neophobia, problem solving, Logan 2016) linked to their learning ability. They will use a colour-reward reinforcement experimental approach to compare the learning performance between male and female great-tailed grackles in three study sites and evaluate whether sex-biased learning ability interacts with sex-biased dispersal. Data will be analysed by a Bayesian reinforcement learning model (Deffner et al. 2020), which was validated. This Stage 1 registered report was evaluated over one round of in-depth review by Jean-François Gerard, Rachel Harrison and one anonymous reviewer, and another round of review by Jean-François Gerard and Rachel Harrison. Based on detailed responses to the comments and the modifications brought to the manuscript by the authors, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA). Withdrawal notice: The Stage 2 manuscript associated with this accepted Stage 1 protocol was submitted to PCI RR on 22 July 2022. On 25 July 2022, the Managing Board offered the opportunity for the authors to revise the manuscript prior to in-depth review. On 7 Sep 2022, the authors withdrew the Stage 2 manuscript from consideration due to time constraints.URL to the preregistered Stage 1 protocol: https://osf.io/v3wxb
Level of bias control achieved: Level 2. At least some data/evidence that will be used to answer the research question has been accessed and partially observed by the authors, but the authors certify that they have not yet observed the key variables within the data that will be used to answer the research question AND they have taken additional steps to maximise bias control and rigour.
List of eligible PCI RR-friendly journals:
References Trochet, A., Courtois, E. A., Stevens, V. M., Baguette, M., Chaine, A., Schmeller, D. S., Clobert, J., & Wiens, J. J. (2016). Evolution of sex-biased dispersal. The Quarterly Review of Biology, 91(3), 297–320. https://doi.org/10.1086/688097 Lee, V. E., & Thornton, A. (2021). Animal cognition in an urbanised world. Frontiers in Ecology and Evolution, 9, 120. https://doi.org/10.3389/fevo.2021.633947 Logan, C. J. (2016b). Behavioral flexibility in an invasive bird is independent of other behaviors. PeerJ, 4, e2215. https://doi.org/10.7717/peerj.2215 Deffner, D., Kleinow, V., & McElreath, R. (2020). Dynamic social learning in temporally and spatially variable environments. Royal Society Open Science, 7(12), 200734. https://doi.org/10.1098/rsos.200734 Breen, A. J. & Deffner D. (2022). Investigating sex differences in learning in a range-expanding bird., https://github.com/alexisbreen/Sex-differences-in-grackles-learning, in principle acceptance of version 2 by Peer Community in Registered Reports. https://osf.io/v3wxb | [WITHDRAWN]: Investigating sex differences in learning in a range-expanding bird | Alexis J. Breen & Dominik Deffner | <p style="text-align: justify;">How might differences in dispersal and learning interact in range expansion dynamics? To begin to answer this question, in this preregistration we detail the background, hypothesis plus associated predictions, and m... | | Life Sciences | Benoit Pujol | Rachel Harrison, Kate Cross, Jean-François Gerard | 2021-11-10 13:12:04 | |
Phenomenological Strands for Gaming Disorder and Esports Play: A Qualitative Registered ReportThe lived experience of gamers: a comparative qualitative investigation of treatment-seekers and esports playersRecommended by Chris Chambers based on reviews by Malte Elson and Peter Branney
Since 2018, the inclusion of “gaming disorder” in the ICD-11 has been met with a mixture of interest, confusion and controversy (Van Rooij et al, 2018), mirroring broader debates about the effects of gaming on mental health. One of the major gaps in understanding the validity of gaming disorder as an identifiable mental illness is the absence of qualitative studies comparing the lived experience of gamers who seek treatment with esports players who do not report health problems.
Here, Karhulahti et al. (2022) tackle this question in the first of two Stage 2 Registered Reports associated with their previous programmatic Stage 1 submission. Using interpretative phenomenological analysis in gamers and medical experts, they find that treatment-seekers and esports players differ in how gaming is associated with the sense of self, either interfering with the self for treatment-seekers or successfully integrating into the self for esports players. These findings help to identify the key characteristics of problematic and non-problematic gaming and call for more intensive and wide-reaching qualitative research in this area.
Following one round of in-depth review and revision, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation.
URL to the preregistered Stage 1 protocol: https://osf.io/a2rwg
Level of bias control achieved: Level 4. At least some of the data/evidence that was used to answer the research question existed prior to in-principle acceptance(IPA) but the authors certify that they did not access any part of that data/evidence prior to IPA.
List of eligible PCI RR-friendly journals:
References
1. van Rooij AJ, Ferguson CJ, Carras MC, Kardefelt-Winther D, Shi J, Aarseth E, Bean AM, Bergmark KH, Brus A, Coulson M, Deleuze J, Dullur P, Dunkels E, Edman J, Elson M, Etchells PJ, Fiskaali A, Granic I, Jansz J, Karlsen F, Kaye LK, Kirsh B, Lieberoth A, Markey P, Mills KL, Nielsen RKL, Orben A, Poulsen A, Prause N, Prax P, Quandt T, Schimmenti A, Starcevic V, Stutman G, Turner NE, Looy J van, Przybylski AK (2018) A weak scientific basis for gaming disorder: Let us err on the side of caution. Journal of Behavioral Addictions, 7, 1–9. https://doi.org/10.1556/2006.7.2018.19
2. Karhulahti V-M, Siutila M, Vahlo J, Koskimaa R (2022) Phenomenological Strands for Gaming Disorder and Esports Play: A Qualitative Registered Report. Stage 2 Registered Report, acceptance of Version 2 by Peer Community in Registered Reports. https://doi.org/10.31234/osf.io/q53jz
| Phenomenological Strands for Gaming Disorder and Esports Play: A Qualitative Registered Report | Veli-Matti Karhulahti, Miia Siutila, Jukka Vahlo, Raine Koskimaa | <p>The recent inclusion of gaming disorder in the ICD-11 as a mental disorder has further increased the importance of researching the health spectrum related to gaming. A critical area in this regard is the lack of clarity concerning the differenc... |  | Medical Sciences, Social sciences | Chris Chambers | 2022-06-11 23:49:03 | ||
05 Jun 2024
STAGE 1
Dose-response of tDCS effects on motor learning and cortical excitability: a preregistered studyHow stimulation intensity affects motor learningRecommended by Christina Artemenko based on reviews by Charlotte Wiltshire and 1 anonymous reviewer
In neurostimulation research, the parameters of a stimulation protocol crucially impact on the effects of the stimulation. Transcranial direct current stimulation (tDCS) is a neurostimulation technique that typically uses current intensities about 1-2 mA in human research to modulate motor and cognitive behavior. The current sham-controlled study by Hsu et al. (2024) applies current intensities not only of 2 mA but also of 4 mA and 6 mA and thus extends our understanding of stimulation parameters while ethical standards are preserved.
The influence of tDCS over the primary motor cortex will be evaluated for neural plasticity during motor learning. Stimulation effects will be tested not only behaviorally but also physiologically by motor evoked potentials elicited by transcranial magnetic stimulation (TMS). The presented pilot data are promising and underline the feasibility of the proposed research design. The study will contribute to tDCS research by uncovering reasons for controversial findings and thus increase reproducibility.
The Stage 1 manuscript was evaluated over two rounds of in-depth review. Based on detailed responses to the reviewers' comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/jyuev
Level of bias control achieved: Level 2. At least some data/evidence that will be used to answer the research question has been accessed and partially observed by the authors, but the authors certify that they have not yet observed the key variables within the data that will be used to answer the research question. List of eligible PCI RR-friendly journals: References
Hsu, G., Edwards, D. J., Cohen, L. G., & Parra, L. C. (2024). Dose-response of tDCS effects on motor learning and cortical excitability: a preregistered study. In principle acceptance of Version 1.3 by Peer Community in Registered Reports. https://osf.io/jyuev
| Dose-response of tDCS effects on motor learning and cortical excitability: a preregistered study | Gavin Hsu, Dylan J. Edwards, Leonardo G. Cohen, Lucas C. Parra | <p>Neuromodulatory effects of transcranial direct current stimulation (tDCS) on the primary motor cortex (M1) have been reported in terms of changes in corticospinal excitability using motor evoked potentials (MEPs), as well as behavioral effects ... | | Engineering, Life Sciences | Christina Artemenko | 2024-01-11 00:11:23 | ||
30 May 2024
STAGE 1
The role of resource dynamics in the distribution of life cycles within a female human populationAn agent-based model of the role of resource dynamics and the environment in human female life cyclesRecommended by Claudio Tennie based on reviews by Cecilia Padilla-Iglesias and 1 anonymous reviewer
Among primates, the human female life cycle appears special. Aspects of these life cycles have been linked to the acquisition and distribution of resources and to environmental factors, as well as to individual differences across human females. Many questions remain regarding the causal roles that these (or also other) factors might have played in the evolution of human female life cycles – and also whether generalizing statements about these life cycles can adequately capture the wide range of the observed phenomena.
In the current study, Varas Enriquez et al. (2024) outline a plan for an agent-based model approach to study the factors that guide and channel variability in female life cycles in humans (within biological constraints), via the effects that their model will capture. The authors’ model has a particular eye towards the effects of resource dynamics (resource production and resource transfers) and environmental conditions – and their interplay. The results of this agent based model will be thoroughly analysed to better understand the evolution of the specific female human life cycle range.
The study plan was refined after one round of review, which led to input from two external reviewers and the recommender. The revised (second) version was judged to satisfy the Stage 1 criteria for in-principle acceptance.
URL to the preregistered Stage 1 protocol: https://osf.io/24c7z
Level of bias control achieved: Level 2. At least some data/evidence that will be used to answer the research question has been accessed and partially observed by the authors, but the authors certify that they have not yet observed the key variables within the data that will be used to answer the research question AND they have taken additional steps to maximise bias control and rigour (e.g. conservative statistical threshold; recruitment of a blinded analyst; robustness testing, multiverse/specification analysis, or other approach) List of eligible PCI RR-friendly journals: References
Varas Enríquez, P. J., Lukas, D., Colleran, H, Mulder, M. B., & Redhead, D. (2024) The role of resource dynamics in the distribution of life cycles within a female human population. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/24c7z
| The role of resource dynamics in the distribution of life cycles within a female human population | Pablo J. Varas Enríquez, Daniel Redhead, Monique Borgerhoff Mulder, Heidi Colleran, Dieter Lukas | <p>The evolution of the female human life cycle, which is characterised by having a reproductive career nested within juvenile and post-reproductive periods, has been linked to the surplus of adult resource production and downwards inter-generatio... | | Life Sciences, Medical Sciences, Social sciences | Claudio Tennie | 2023-11-13 15:45:52 | ||
22 Jul 2024
STAGE 1
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”Replicating, Revising and Reforming: Unpicking the Apparent Nanoparticle Endosomal Escape ParadoxRecommended by Emily Linnane and Yuki Yamada based on reviews by Cecilia Menard-Moyon and Zeljka Krpetic
Context
Over the past decade there has been an exponential increase in the number of research papers utlising nanoparticles for biological applications such as intracellular sensing [1, 2], theranostics [3-5] and more recently drug delivery and precision medicine [6, 7]. Despite the success stories, there is a disconnect regarding current dogma on issues such as nanoparticle uptake and trafficking, nanoparticle delivery via the enhanced permeability and retention (EPR) effect, and endosomal escape. Critical re-evaluation of these concepts both conceptually and experimentally is needed for continued advancement in the field.
For this preregistration, Said et al. (2024) focus on nanoparticle intracellular trafficking, specifically endosomal escape [8]. The current consensus in the literature is that nanoparticles enter cells via endocytosis [9, 10] but reportedly just 1-2% of nanoparticles/ nanoparticle probes escape endosomes and enter the cytoplasm [11-13]. There is therefore an apparent paradox over how sensing nanoparticles can detect their intended targets in the cytoplasm if they are trapped within the cell endosomes. To address this fundamental issue of nanoparticle endosomal escape, Lévy and coworkers are carrying out replication studies to thoroughly and transparently replicate the most influential papers in the field of nanoparticle sensing. The aim of these replication studies is twofold: to establish a robust methodology to study endosomal escape of nanoparticles, and to encourage discussions, transparency and a step-change in the field.
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”
For this replication study, the authors classified papers on the topic of nanoparticle sensing and subsequently ranked them by number of citations. Based on this evaluation they selected a paper by Zhu and colleagues [14] entitled “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions” for their seminal replication study. To determine the reproducibility of the results from Zhu et al., the authors aim to establish the proportion of endosomal escape of the nanoparticles, and to examine the data in a biological context relevant to the application of the probe.
Beyond Replication
The authors plan to replicate the exact conditions reported in the materials and methods section of the selected paper such as nanoparticle probe synthesis of CdSe@C-TPEA nanoparticles, assessment of particle size, stability and reactivity and effect on cells (TEM, pH experiments, fluorescent responsivity to metal ions and cell viability). In addition, Said et al., plan to include further experimental characterisation to complement the existing study by Zhu and colleagues. They will incorporate additional controls and methodology to determine the intracellular location of nanoparticle probes in cells including: quantifying excess fluorescence in the culture medium, live cell imaging analysis, immunofluorescence with endosomal and lysosomal markers, and electron microscopy of cell sections. The authors will also include supplementary viability studies to assess the impact of the nanoparticles on HeLa cells as well as an additional biologically relevant cell line (for use in conjunction with the HeLa cells as per the original paper).
The Stage 1 manuscript underwent two rounds of thorough in-depth review. After considering the detailed responses to the reviewers' comments, the recommenders determined that the manuscript met the Stage 1 criteria and awarded in-principle acceptance (IPA).
The authors have thoughtfully considered their experimental approach to the replication study, whilst acknowledging any potential limitations. Given that conducting such a replication study is novel in the field of Nanotechnology and there is currently no ‘gold standard’ approach in doing so, the authors have showed thoughtful regard of statistical analysis and unbiased methodology where possible.
Based on current information, this study is the first use of preregistration via Peer Community in Registered Reports and the first formalised replication study in Nanotechnology for Biosciences. The outcomes of this of this study will be significant both scientifically and in the wider context in discussion of the scientific method.
URL to the preregistered Stage 1 protocol: https://osf.io/qbxpf
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly Journals:
References
1. Howes, P. D., Chandrawati, R., & Stevens, M. M. (2014). Colloidal nanoparticles as advanced biological sensors. Science, 346(6205), 1247390. https://doi.org/10.1126/science.1247390
2. Liu, C. G., Han, Y. H., Kankala, R. K., Wang, S. B., & Chen, A. Z. (2020). Subcellular performance of nanoparticles in cancer therapy. International Journal of Nanomedicine, 675-704. https://doi.org/10.2147/IJN.S226186
3. Tang, W., Fan, W., Lau, J., Deng, L., Shen, Z., & Chen, X. (2019). Emerging blood–brain-barrier-crossing nanotechnology for brain cancer theranostics. Chemical Society Reviews, 48(11), 2967-3014. https://doi.org/10.1039/C8CS00805A
4. Yoon, Y. I., Pang, X., Jung, S., Zhang, G., Kong, M., Liu, G., & Chen, X. (2018). Smart gold nanoparticle-stabilized ultrasound microbubbles as cancer theranostics. Journal of Materials Chemistry B, 6(20), 3235-3239. https://doi.org/10.1039%2FC8TB00368H
5. Lin, H., Chen, Y., & Shi, J. (2018). Nanoparticle-triggered in situ catalytic chemical reactions for tumour-specific therapy. Chemical Society Reviews, 47(6), 1938-1958. https://doi.org/10.1039/C7CS00471K
6. Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021). Lipid nanoparticles for mRNA delivery. Nature Reviews Materials, 6(12), 1078-1094. https://doi.org/10.1038/s41578-021-00358-0
7. Mitchell, M. J., Billingsley, M. M., Haley, R. M., Wechsler, M. E., Peppas, N. A., & Langer, R. (2021). Engineering precision nanoparticles for drug delivery. Nature Reviews Drug Discovery, 20(2), 101-124. https://doi.org/10.1038/s41573-020-0090-8
8. Said, M., Gharib, M., Zrig, S., & Lévy, R. (2024). Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/qbxpf
9. Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M. A., Alkawareek, M. Y., Dreaden, E. C., ... & Mahmoudi, M. (2017). Cellular uptake of nanoparticles: Journey inside the cell. Chemical Society Reviews, 46(14), 4218-4244. https://doi.org/10.1039/C6CS00636A
10. de Almeida, M. S., Susnik, E., Drasler, B., Taladriz-Blanco, P., Petri-Fink, A., & Rothen-Rutishauser, B. (2021). Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine. Chemical society reviews, 50(9), 5397-5434. https://doi.org/10.1039/D0CS01127D
11. Smith, S. A., Selby, L. I., Johnston, A. P., & Such, G. K. (2018). The endosomal escape of nanoparticles: toward more efficient cellular delivery. Bioconjugate Chemistry, 30(2), 263-272. http://dx.doi.org/10.1021/acs.bioconjchem.8b00732
12. Cupic, K. I., Rennick, J. J., Johnston, A. P., & Such, G. K. (2019). Controlling endosomal escape using nanoparticle composition: current progress and future perspectives. Nanomedicine, 14(2), 215-223. https://doi.org/10.2217/nnm-2018-0326
13. Wang, Y., & Huang, L. (2013). A window onto siRNA delivery. Nature Biotechnology, 31(7), 611-612. https://doi.org/10.1038/nbt.2634
14. Zhu, A., Qu, Q., Shao, X., Kong, B., & Tian, Y. (2012). Carbon-dot-based dual-emission nanohybrid produces a ratiometric fluorescent sensor for in vivo imaging of cellular copper ions. Angewandte Chemie (International ed. in English), 51(29), 7185-7189. https://doi.org/10.1002/anie.201109089
| Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions” | Maha Said, Mustafa Gharib, Samia Zrig, Raphaël Lévy | <p>In hundreds of articles published over the past two decades, nanoparticles have been described as probes for sensing and imaging of a variety of intracellular cytosolic targets. However, nanoparticles generally enter cells by endocytosis with o... | | Life Sciences, Medical Sciences, Physical Sciences | Emily Linnane | 2023-11-29 19:14:03 | ||
Progression of white matter hyperintensities is related to blood pressure increases and global cognitive decline – a registered reportWhite matter lessions are associated with increases in blood pressure and global cognitive declineRecommended by Chris Chambers based on reviews by Isabel Garcia Garcia
Cerebral small vessel disease (CSVD) is a common and multi-faceted set of pathologies that affect the small arteries, arterioles, venules and capillaries of the brain. The disease manifests through a range of symptoms and conditions, including psychiatric disorders, abnormal gait, and urinary incontinence, while accounting for 25% of strokes and nearly 50% of dementia.
The presence of CSVD is associated with white matter lesions detected as white matter hyperintensities (WMH) using neuroimaging, which have in turn been shown to predict future stroke, cognitive decline and dementia. While vascular risk factors of CSVD (such as hypertension and obesity) are also associated with CSVD, a complete picture of the predictive relationship between WMH, cognitive decline, and blood pressure remains to be determined, as does the role of sex/gender. These inter-relationships are important to determine for improving the diagnosis and treatment of CSVD.
In the current study, Beyer et al. analysed a large emerging dataset from the LIFE-Adult project – a longitudinal, two-wave, population-based study – to ask whether higher blood pressure predicts a greater increase in WMH, and whether progression of WMH is associated with measures of memory and executive function. In addition, the authors explored the relationship between abdominal obesity and WMH progression, and the extent to which WMH progression, and its interaction with vascular risk factors, depends on sex/gender.
Results revealed no reliable association between baseline blood pressure with WMH progression. WMH progression significantly predicted global cognitive decline but not decline in executive function specifically. Exploratory analyses revealed that increases in diastolic blood pressure as well as baseline and systolic blood pressure were associated with WMH progression, specifically in frontal periventricular regions, but there was no association of waist-to-hip ratio (a proxy of abdominal fat deposits) with WMH progression nor any gender-specific associations. The authors conclude that strict control of blood pressure might confer a protective effect, limiting WMH progression and negative effects on global cognitive function in the middle-aged to older population.
The Stage 2 manuscript was evaluated over one round of in-depth review. Based on responses to the reviewer's comments, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation.
URL to the preregistered Stage 1 protocol: https://osf.io/qkbgj Level of bias control achieved: Level 2. At least some data/evidence that was used to answer the research question had been accessed and partially observed by the authors prior to Stage 1 in-principle acceptance, but the authors certify that they had not yet observed the key variables within the data that were used to answer the research question.
List of eligible PCI RR-friendly journals: References
1. Beyer, F., Lammer, L., Loeffler, M., Riedel-Heller, S., Debette, S., Villringer, A. & Witte, A. V. (2023). Progression of white matter hyperintensities is related to blood pressure increases and global cognitive decline – a registered report [Stage 2]. Acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/k24pm
| Progression of white matter hyperintensities is related to blood pressure increases and global cognitive decline – a registered report | Frauke Beyer, Laurenz Lammer, Markus Loeffler, Steffi Riedel-Heller, Stéphanie Debette, Arno Villringer, A. Veronica Witte | <p>Introduction<br>White matter hyperintensities (WMH) reflect cerebral small vessel disease (cSVD), a major brain pathology contributing to cognitive decline and dementia. Vascular risk factors including higher diastolic blood pressure (DBP) have... |  | Humanities, Medical Sciences | Chris Chambers | 2024-02-15 17:16:37 | ||
27 Mar 2023
STAGE 1
Optimizing Esports Performance Using a Synergistic Mindsets InterventionSynergistic Mindset Intervention for Competitive SituationsRecommended by Veli-Matti Karhulahti based on reviews by Lee Moore, Ivan Ropovik , Ivana Piterová and Jacob Keech
Mindset theories suggest that the mere belief in the malleability of human abilities can already help one to develop related performance. On the other hand, one and the same performance situation can also be experienced in various affective ways, which differently contribute to performance outcomes. Arguably, appraising a performance situation as a “threat” instead of “challenge” is associated with maladaptive responses, such as impaired cardiovascular mobilization. If people could experience performance situations as positive challenges, this might also improve performance outcomes. Drawing from these connected theoretical premises, the synergistic mindset intervention was developed and tentatively found to help adolescents in stressful situations (Yeager et al., 2022).
In the present registered report, Behnke et al. (2023) build on the above and test whether the synergistic mindset intervention can help individuals in competitive gaming situations. The authors utilize one of the leading esport games, Counter-Strike: Global Offensive, and recruit its active players into randomized control and intervention groups for two weeks. Ultimately, the participants compete in a cash-prize tournament involving measures of affective experience and cardiovascular responses. Behnke et al. (2023) hypothesize that the synergistic mindset group will show greater challenge affective responses and superior performance outcomes. As such, the study design has significant potential to generate valuable evidence for various theoretical models and the synergistic mindset model in particular. The Stage 1 manuscript was evaluated over two rounds by four experts with experimental psychology specializations in mindsets, stress, and statistics. Based on the comprehensive responses to the reviewers' feedback, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA). URL to the preregistered Stage 1 protocol: https://osf.io/z3adb Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly journals:
References
Behnke M., Lakens D., Petrova K., Chwiłkowska P., Kaczmarek L. D., Jamieson J. P., & Gross J. J. (2023) Optimizing Esports Performance Using a Synergistic Mindsets Intervention. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/z3adb Yeager D.S., Bryan C.J., Gross J.J., Murray J., Krettek D., Santos P., ... & Jamieson J.P. (2022) A synergistic mindsets intervention protects adolescents from stress. Nature 607, 512–520. https://doi.org/10.1038/s41586-022-04907-7 | Optimizing Esports Performance Using a Synergistic Mindsets Intervention | Maciej Behnke, Daniël Lakens, Kate Petrova, Patrycja Chwiłkowska, Lukasz D. Kaczmarek, Jeremy P. Jamieson, James J. Gross | <p>Affective responses during stressful high-stakes situations can play an important role in shaping performance outcomes. For example, feeling shaky and nervous at a job interview can undermine performance, whereas feeling pumped and excited duri... | | Social sciences | Veli-Matti Karhulahti | 2023-01-04 10:12:55 |
