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Latest recommendations
| Id | Title * | Authors * | Abstract * | Picture▼ | Thematic fields * | Recommender | Reviewers | Submission date | |
|---|---|---|---|---|---|---|---|---|---|
Is the past farther than the future? A registered replication and test of the time-expansion hypothesis based on the filling rate of durationThe Temporal Doppler Effect may not be a robust and culturally universal phenomenonRecommended by Ljerka Ostojic based on reviews by Chris Chambers and 1 anonymous reviewer
The Temporal Doppler Effect refers to the subjective perception that the past is further away than the future even when both temporal distances are objectively the same from the present moment (Caruso et al., 2013). In the current study, Zhang et al. ran a replication of this phenomenon and tested one possible explanation for it, namely that people overestimate the temporal distance of the past because the past is filled with more events than the future. This is because we can access information only about planned events for the future, but have access to both planned and unplanned events that happened in the past (filled-duration illusion; Thomas & Brown, 1974).
Over two studies, the authors found that the sampled participants reported feeling that the past was psychologically closer than the future, which is the opposite of what has previously been reported and termed the Temporal Doppler Effect (Caruso et al., 2013). In addition, the authors reported inconsistent results regarding the correlations between the psychological distance and different variables associated with the filling rate of duration. The authors discuss the differences between their own results and those by Caruso et al. (2013) in terms of methodological and contextual differences and highlight cultural aspects that may be critical to consider in future replications and overall testing of this phenomenon. As such, they highlight that, at the moment, the Temporal Doppler Effect should not be considered a robust and culturally universal phenomenon.
The Stage 2 manuscript was evaluated by two reviewers who had also reviewed the stage 1 report. Following a revision by the authors, which consisted of adding the Data Availability statement, as well as a more precise summary of the results in various sections of the report, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation.
URL to the preregistered Stage 1 protocol: https://osf.io/d9ec3/
Level of bias control achieved: Level 6. No part of the data or evidence that was used to answer the research question existed prior to Stage 1 in-principle acceptance. List of eligible PCI RR-friendly journals:
References
1. Caruso, E. M., Van Boven, L., Chin, M., & Ward, A. (2013). The temporal doppler effect: When the future feels closer than the past. Psychological Science, 24, 530-536. https://doi.org/10.1177/0956797612458804
2. Thomas, E. C., & Brown, I. (1974). Time perception and the filled-duration illusion. Perception & Psychophysics, 16, 449-458. https://doi.org/10.3758/BF03198571
3. Zhang, Q., Masuda, Y., Ueda, K.,Toda, K., & Yamada, Y. (2022). Is the past farther than the future? A registered replication and test of the time-expansion hypothesis based on the filling rate of duration. Stage 2 Registered Report, acceptance of Version 2 by Peer Community in Registered Reports. https://psyarxiv.com/pb47n/
| Is the past farther than the future? A registered replication and test of the time-expansion hypothesis based on the filling rate of duration | Qinjing Zhang, Yoshitaka Masuda, Kodai Toda, Kohei Ueda, Yuki Yamada | <p>People feel some events to be psychologically closer, while others to be farther away. Caruso et al. (2013) reported the Temporal Doppler Effect (TDE), in which people feel that the past is farther than the future, despite an equivalent objecti... |  | Social sciences | Ljerka Ostojic | 2022-08-20 09:59:09 | ||
25 Mar 2024
STAGE 1
Assessing compliance with UK loot box industry self-regulation on the Apple App Store: a 6-month longitudinal study on the implementation processDoes self regulation by gaming companies for the use of loot boxes work?Recommended by Zoltan Dienes based on reviews by Chris Chambers, Lukas J. Gunschera and Andy Przybylski
Video games may provide the option of spending real money in exchange for probabilistically receiving game-relevant rewards; in effect, encouraging potentially young teenagers to gamble. The industry has subscribed to a set of regulatory principles to cover the use of such "loot boxes", including 1) that they will prevent loot box purchasing by under 18s unless parental consent is given; 2) that they will make it initially clear that the game contains loot boxes; and 3) that they will clearly disclose the probabilities of receiving different rewards.
Can the industry effectively self regulate? Xiao (2024) will evaluate this important question by investigating the 100 top selling games on the Apple App Store and estimating the percentage compliance to these three regulatory principles at two time points 6 months apart.
The Stage 1 manuscript was evaluated over one round of in-depth review. Based on detailed responses to the reviewers' comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/3knyb
Level of bias control achieved: Level 2. At least some data/evidence that will be used to answer the research question has been accessed and partially observed by the authors, but the authors certify that they have not yet observed the key variables within the data that will be used to answer the research question. List of eligible PCI RR-friendly journals:
References 1. Xiao, L. (2024). Assessing compliance with UK loot box industry self-regulation on the Apple App Store: a 6-month longitudinal study on the implementation process. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/3knyb | Assessing compliance with UK loot box industry self-regulation on the Apple App Store: a 6-month longitudinal study on the implementation process | Leon Y. Xiao | <p>Loot boxes in video games can be purchased with real-world money in exchange for random rewards. Stakeholders are concerned about loot boxes’ similarities with gambling and their potential harms (e.g., overspending). The UK Government has decid... | | Humanities, Social sciences | Zoltan Dienes | 2023-08-27 22:47:03 | ||
Dose-response of tDCS effects on motor learning and cortical excitability: a preregistered studyIncreasing stimulation intensity does not affect motor learningRecommended by Christina Artemenko based on reviews by 1 anonymous reviewer
In neurostimulation research, the parameters of a stimulation protocol crucially impact on the effects of the stimulation. Transcranial direct current stimulation (tDCS) is a neurostimulation technique that typically uses current intensities about 1-2 mA in human research to modulate motor and cognitive behavior. The current sham-controlled study by Hsu et al. (2024) applies current intensities not only of 2 mA but also of 4 mA and 6 mA and thus extends our understanding of stimulation parameters while ethical standards are preserved.
The influence of tDCS over the primary motor cortex was evaluated for neural plasticity during motor learning. Stimulation effects were tested not only behaviorally but also physiologically by motor evoked potentials elicited by transcranial magnetic stimulation (TMS). The presented pilot data were promising and underlined the feasibility of the proposed research design. The study contributes to tDCS research by uncovering reasons for controversial findings and thus increases reproducibility.
The results of the study unexpectedly revealed no stimulation effects on motor learning, neither for behavioral outcomes nor for physiological outcomes by motor evoked potentials. No evidence was found that stimulation effects linearly increase with increasing intensity. Interestingly, higher intensities were relatively well tolerated - but did not have any impact. The current findings underline the purpose of preregistrations and registered reports to act against publication bias, particulary in the field of neuromodulation. In the current case, failed replication and null findings - revealed by a methodologically sound study - are crucial to inform future research using similar stimulation protocols with the aim to modulate motor or cognitive behavior.
The Stage 2 manuscript was evaluated over one round of review. Based on detailed responses to reviewers’ and the recommender’s comments, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation.
URL to the preregistered Stage 1 protocol: https://osf.io/jyuev
Level of bias control achieved: Level 2. At least some data/evidence that was used to answer the research question had been accessed and partially observed by the authors prior to Stage 1 in-principle acceptance, but the authors certify that they had not yet observed the key variables within the data that were used to answer the research question. List of eligible PCI RR-friendly journals: References
Hsu, G., Jafari, Z. H., Ahmed, A., Edwards, D. J., Cohen, L. G., & Parra, L. C. (2024). Dose-response of tDCS effects on motor learning and cortical excitability: a preregistered study [Stage 2]. Acceptance of Version 2.1 by Peer Community in Registered Reports. https://osf.io/a42uy
| Dose-response of tDCS effects on motor learning and cortical excitability: a preregistered study | Gavin Hsu, Zhenous Hadi Jafari, Abdelrahman Ahmed, Dylan J. Edwards, Leonardo G. Cohen, Lucas C. Parra | <p>Background: Multiple studies have demonstrated that transcranial direct current stimulation (tDCS) of the primary motor cortex (M1) can influence corticospinal excitability and motor skill acquisition. However, the evidence for these effects i... |  | Engineering, Medical Sciences | Christina Artemenko | 2024-09-02 19:07:02 | ||
17 Jan 2022
STAGE 1
Identifying Gaming Disorders by Ontology: A Nationally Representative Registered ReportDo different screening instruments for ‘gaming disorder’ measure the same or different construct(s)?Recommended by Charlotte Pennington based on reviews by Daniel Dunleavy, Linda Kaye, David Ellis and 1 anonymous reviewer
There is considerable debate regarding the relationship between excessive gaming and mental health problems. Whilst the diagnostic classification of “gaming disorder” has now been included in the WHO’s International Classification of Diseases (ICD-11), the APA decided not to include this diagnosis in their Diagnostic and Statistical Manual of Mental Disorders (DSM-5) because the literature “suffers from a lack of a standard definition from which to derive prevalence data” (APA 2013, p. 796). Furthermore, screening instruments that aim to provide diagnostic classifications derive from different ontologies and it is not known whether they identify equivalent prevalence rates of ‘gaming disorder’ or even the same individuals. In this Stage 1 Registered Report, Karhulahti et al. (2022) aim to assess how screening instruments that derive from different ontologies differ in identifying associated problem groups. A nationally representative sample of 8000 Finnish individuals will complete four screening measures to assess the degree of overlap between identified prevalence (how many?), who they identify (what characteristics?) and the health of their identified groups (how healthy?). If these four ontologically diverse instruments operate similarly, this will support the notion of a single “gaming disorder” construct. If, however, the instruments operate differently, this will suggest that efforts should be directed toward assessing the clinical (ir)relevance of multiple constructs. This rigorous study will therefore have important implications for the conceptualisation and measurement of “gaming disorder”, contributing to the debate around the mixed findings of gaming-related health problems. Four expert reviewers with field expertise assessed the Stage 1 manuscript over three rounds of in-depth review. Based on detailed and informed responses to the reviewers' comments, the recommender decided that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA). URL to the preregistered Stage 1 protocol: https://osf.io/usj5b Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly journals:
References
| Identifying Gaming Disorders by Ontology: A Nationally Representative Registered Report | Veli-Matti Karhulahti, Jukka Vahlo, Marcel Martončik, Matti Munukka, Raine Koskimaa, Mikaela von Bonsdorff | <p style="text-align: justify;">Gaming-related health problems have been researched since the 1980s with numerous different “ontologies” as reference systems, from self-assessed “game addiction” to “pathological gambling” (in the DSM-IV), “interne... | | Medical Sciences, Social sciences | Charlotte Pennington | 2021-08-25 23:08:26 | ||
Impact of analytic decisions on test-retest reliability of individual and group estimates in functional magnetic resonance imaging: a multiverse analysis using the monetary incentive delay taskExploring determinants of test-retest reliability in fMRI: a study with the Monetary Incentive Delay TaskRecommended by Dorothy Bishop based on reviews by Xiangzhen Kong and 1 anonymous reviewer
Functional magnetic resonance imaging has been used to explore brain-behaviour relationships for many years, with proliferation of a wide range of sophisticated analytic procedures. However, rather scant attention has been paid to the reliability of findings. Concerns have been growing failures to replicate findings in some fields, but it is hard to know how far this is a consequence of underpowered studies, flexible analytic pipelines, or variability within and between participants. Demidenko et al. (2024) took advantage of the availability of three existing datasets, including the Adolescent Brain Cognitive Development (ABCD) study, the Michigan Longitudinal Study, and the Adolescent Risk Behavior Study, which all included a version of the Monetary Incentive Delay task measured in two sessions. These were entered into a multiverse analysis, which considered how within-subject and between-subject variance varies according to four analytic factors: smoothing (5 levels), motion correction (6 levels), task modelling (3 levels) and task contrasts (4 levels). They also considered how sample size affects estimates of reliability. The results have important implications for the those using fMRI with the Monetary Incentive Delay Task, and also raise questions more broadly about use of fMRI indices to study individual differences. Motion correction had relatively little impact on the ICC, and the effect size of the smoothing kernel was modest. Larger impacts on reliability were associated with choice of contrast (implicit baseline giving larger effects) and task parameterization. But perhaps the most sobering message from this analysis is that although activation maps from group data were reasonably reliable, the ICC, used as an index of reliability for individual levels of activation, was consistently low. This raises questions about the suitability of the Monetary Incentive Delay Task for studying individual differences. Another point is that reliability estimates become more stable as sample size increases; researchers may want to consider whether the trade-off between cost and gain in precision is justified for sample sizes above 250. I did a quick literature search on Web of Science: at the time of writing the search term ("Monetary Delay Task" AND fMRI) yielded 410 returns, indicating that this is a popular method in cognitive neuroscience. The detailed analyses reported here will repay study for those who are planning further research using this task. The Stage 2 manuscript was evaluated over one round of in-depth review. Based on detailed responses to the reviewer's and recommender's comments, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation. URL to the preregistered Stage 1 protocol: https://osf.io/nqgeh
Level of bias control achieved: Level 2. At least some data/evidence that was used to answer the research question had been accessed and partially observed by the authors prior to IPA, but the authors certify that they had not yet sufficiently observed the key variables within the data to be able to answer the research questions and they took additional steps to maximise bias control and rigour.
List of eligible PCI RR-friendly journals:
References 1. Demidenko, M. I., Mumford, J. A., & Poldrack, R. A. (2024). Impact of analytic decisions on test-retest reliability of individual and group estimates in functional magnetic resonance imaging: a multiverse analysis using the monetary incentive delay task [Stage 2]. Acceptance of Version 5 by Peer Community in Registered Reports. https://www.biorxiv.org/content/10.1101/2024.03.19.585755v4 | Impact of analytic decisions on test-retest reliability of individual and group estimates in functional magnetic resonance imaging: a multiverse analysis using the monetary incentive delay task | Michael I. Demidenko, Jeanette A. Mumford, Russell A. Poldrack | <p>Empirical studies reporting low test-retest reliability of individual blood oxygen-level dependent (BOLD) signal estimates in functional magnetic resonance imaging (fMRI) data have resurrected interest among cognitive neuroscientists in methods... |  | Life Sciences, Social sciences | Dorothy Bishop | 2024-03-21 02:23:30 | ||
17 Jan 2023
STAGE 1
Similarities and differences in a global sample of song and speech recordingsExploring cross-cultural variation in speech and songRecommended by Chris Chambers based on reviews by Bob Slevc, Nai Ding and 1 anonymous reviewer
For centuries, the ubiquity of language and music across human societies has prompted scholars to speculate about their cross-cultural origins as well as their shared and unique characteristics. Depending on the extent to which contemporary theories emphasise the role of biology vs. culture, a range of hypotheses have been proposed concerning expected similarities and differences in song and speech. One class of hypotheses stemming from cultural relativism assumes a lack of universal regularities in song and speech, and therefore predicts no systematic cross-cultural relationships. On the other hand, more recent evolutionary hypotheses such as the social bonding hypothesis, motor constraint hypothesis, and sexual selection hypothesis all predict differences or similarities in specific characteristic of vocalisations, such as pitch regularity, pitch interval size, and melodic contour. Existing results are mixed in their support of these predictions.
In the current study, Ozaki et al. (2022) embark on an ambitious project to elucidate cross-cultural similarities and differences between speech and song in 81 different linguistic varieties spanning 23 language families. Understanding precisely how song and speech are related is methodologically challenging due to the multitude of confounds that can arise in comparing natural recordings. Here the authors overcome these difficulties with four types of carefully controlled recordings: singing, recitation of sung lyrics, spoken description of the song, and instrumental version of the sung melody. The authors will then examine six features that are amenable to reliable comparison, including pitch height, temporal rate, pitch stability, timbral brightness, pitch interval size, and pitch declination. With this data in hand, the authors will ask which acoustic features differ reliably between song and speech across cultures, with the expectation that song will exhibit higher pitch, slower rate and more stable pitch than speech. At the same time, the authors expect song and speech to be reliably similar in the characteristics of timbral brightness, pitch intervals and pitch contours. In addition to these confirmatory tests, the authors will explore variation across a range of additional stimulus characteristics and ancillary research questions.
The Stage 1 manuscript was evaluated over two rounds of in-depth review. Based on detailed responses to the reviewers' comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
URL to the preregistered Stage 1 protocol: https://osf.io/jdhtz
Level of bias control achieved: Level 2. At least some data/evidence that will be used to answer the research question has been accessed and partially observed by the authors, but the authors certify that they have not yet observed the key variables within the data that will be used to answer the research question AND they have taken additional steps to maximise bias control and rigour.
List of eligible PCI RR-friendly journals: References
1. Ozaki, Y., Savage P. E. et al. (2022). Similarities and differences in a global sample of song and speech recordings, in principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/jdhtz
| Similarities and differences in a global sample of song and speech recordings | Corresponding authors: Yuto Ozaki and Patrick E. Savage (Keio University, Japan). Full list of 80 authors is in the manuscript | <p>What, if any, similarities and differences between song and speech are consistent across cultures? Both song and speech are found in all known human societies and are argued to share evolutionary roots and cognitive resources, yet no studies ha... | | Social sciences | Chris Chambers | Nai Ding, Bob Slevc | 2022-09-16 16:03:10 | |
27 Mar 2023
STAGE 1
Optimizing Esports Performance Using a Synergistic Mindsets InterventionSynergistic Mindset Intervention for Competitive SituationsRecommended by Veli-Matti Karhulahti based on reviews by Lee Moore, Ivan Ropovik , Ivana Piterová and Jacob Keech
Mindset theories suggest that the mere belief in the malleability of human abilities can already help one to develop related performance. On the other hand, one and the same performance situation can also be experienced in various affective ways, which differently contribute to performance outcomes. Arguably, appraising a performance situation as a “threat” instead of “challenge” is associated with maladaptive responses, such as impaired cardiovascular mobilization. If people could experience performance situations as positive challenges, this might also improve performance outcomes. Drawing from these connected theoretical premises, the synergistic mindset intervention was developed and tentatively found to help adolescents in stressful situations (Yeager et al., 2022).
In the present registered report, Behnke et al. (2023) build on the above and test whether the synergistic mindset intervention can help individuals in competitive gaming situations. The authors utilize one of the leading esport games, Counter-Strike: Global Offensive, and recruit its active players into randomized control and intervention groups for two weeks. Ultimately, the participants compete in a cash-prize tournament involving measures of affective experience and cardiovascular responses. Behnke et al. (2023) hypothesize that the synergistic mindset group will show greater challenge affective responses and superior performance outcomes. As such, the study design has significant potential to generate valuable evidence for various theoretical models and the synergistic mindset model in particular. The Stage 1 manuscript was evaluated over two rounds by four experts with experimental psychology specializations in mindsets, stress, and statistics. Based on the comprehensive responses to the reviewers' feedback, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA). URL to the preregistered Stage 1 protocol: https://osf.io/z3adb Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly journals:
References
Behnke M., Lakens D., Petrova K., Chwiłkowska P., Kaczmarek L. D., Jamieson J. P., & Gross J. J. (2023) Optimizing Esports Performance Using a Synergistic Mindsets Intervention. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/z3adb Yeager D.S., Bryan C.J., Gross J.J., Murray J., Krettek D., Santos P., ... & Jamieson J.P. (2022) A synergistic mindsets intervention protects adolescents from stress. Nature 607, 512–520. https://doi.org/10.1038/s41586-022-04907-7 | Optimizing Esports Performance Using a Synergistic Mindsets Intervention | Maciej Behnke, Daniël Lakens, Kate Petrova, Patrycja Chwiłkowska, Lukasz D. Kaczmarek, Jeremy P. Jamieson, James J. Gross | <p>Affective responses during stressful high-stakes situations can play an important role in shaping performance outcomes. For example, feeling shaky and nervous at a job interview can undermine performance, whereas feeling pumped and excited duri... | | Social sciences | Veli-Matti Karhulahti | 2023-01-04 10:12:55 | ||
Progression of white matter hyperintensities is related to blood pressure increases and global cognitive decline – a registered reportWhite matter lessions are associated with increases in blood pressure and global cognitive declineRecommended by Chris Chambers based on reviews by Isabel Garcia Garcia
Cerebral small vessel disease (CSVD) is a common and multi-faceted set of pathologies that affect the small arteries, arterioles, venules and capillaries of the brain. The disease manifests through a range of symptoms and conditions, including psychiatric disorders, abnormal gait, and urinary incontinence, while accounting for 25% of strokes and nearly 50% of dementia.
The presence of CSVD is associated with white matter lesions detected as white matter hyperintensities (WMH) using neuroimaging, which have in turn been shown to predict future stroke, cognitive decline and dementia. While vascular risk factors of CSVD (such as hypertension and obesity) are also associated with CSVD, a complete picture of the predictive relationship between WMH, cognitive decline, and blood pressure remains to be determined, as does the role of sex/gender. These inter-relationships are important to determine for improving the diagnosis and treatment of CSVD.
In the current study, Beyer et al. analysed a large emerging dataset from the LIFE-Adult project – a longitudinal, two-wave, population-based study – to ask whether higher blood pressure predicts a greater increase in WMH, and whether progression of WMH is associated with measures of memory and executive function. In addition, the authors explored the relationship between abdominal obesity and WMH progression, and the extent to which WMH progression, and its interaction with vascular risk factors, depends on sex/gender.
Results revealed no reliable association between baseline blood pressure with WMH progression. WMH progression significantly predicted global cognitive decline but not decline in executive function specifically. Exploratory analyses revealed that increases in diastolic blood pressure as well as baseline and systolic blood pressure were associated with WMH progression, specifically in frontal periventricular regions, but there was no association of waist-to-hip ratio (a proxy of abdominal fat deposits) with WMH progression nor any gender-specific associations. The authors conclude that strict control of blood pressure might confer a protective effect, limiting WMH progression and negative effects on global cognitive function in the middle-aged to older population.
The Stage 2 manuscript was evaluated over one round of in-depth review. Based on responses to the reviewer's comments, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation.
URL to the preregistered Stage 1 protocol: https://osf.io/qkbgj Level of bias control achieved: Level 2. At least some data/evidence that was used to answer the research question had been accessed and partially observed by the authors prior to Stage 1 in-principle acceptance, but the authors certify that they had not yet observed the key variables within the data that were used to answer the research question.
List of eligible PCI RR-friendly journals: References
1. Beyer, F., Lammer, L., Loeffler, M., Riedel-Heller, S., Debette, S., Villringer, A. & Witte, A. V. (2023). Progression of white matter hyperintensities is related to blood pressure increases and global cognitive decline – a registered report [Stage 2]. Acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/k24pm
| Progression of white matter hyperintensities is related to blood pressure increases and global cognitive decline – a registered report | Frauke Beyer, Laurenz Lammer, Markus Loeffler, Steffi Riedel-Heller, Stéphanie Debette, Arno Villringer, A. Veronica Witte | <p>Introduction<br>White matter hyperintensities (WMH) reflect cerebral small vessel disease (cSVD), a major brain pathology contributing to cognitive decline and dementia. Vascular risk factors including higher diastolic blood pressure (DBP) have... |  | Humanities, Medical Sciences | Chris Chambers | 2024-02-15 17:16:37 | ||
22 Jul 2024
STAGE 1
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”Replicating, Revising and Reforming: Unpicking the Apparent Nanoparticle Endosomal Escape ParadoxRecommended by Emily Linnane and Yuki Yamada based on reviews by Cecilia Menard-Moyon and Zeljka Krpetic
Context
Over the past decade there has been an exponential increase in the number of research papers utlising nanoparticles for biological applications such as intracellular sensing [1, 2], theranostics [3-5] and more recently drug delivery and precision medicine [6, 7]. Despite the success stories, there is a disconnect regarding current dogma on issues such as nanoparticle uptake and trafficking, nanoparticle delivery via the enhanced permeability and retention (EPR) effect, and endosomal escape. Critical re-evaluation of these concepts both conceptually and experimentally is needed for continued advancement in the field.
For this preregistration, Said et al. (2024) focus on nanoparticle intracellular trafficking, specifically endosomal escape [8]. The current consensus in the literature is that nanoparticles enter cells via endocytosis [9, 10] but reportedly just 1-2% of nanoparticles/ nanoparticle probes escape endosomes and enter the cytoplasm [11-13]. There is therefore an apparent paradox over how sensing nanoparticles can detect their intended targets in the cytoplasm if they are trapped within the cell endosomes. To address this fundamental issue of nanoparticle endosomal escape, Lévy and coworkers are carrying out replication studies to thoroughly and transparently replicate the most influential papers in the field of nanoparticle sensing. The aim of these replication studies is twofold: to establish a robust methodology to study endosomal escape of nanoparticles, and to encourage discussions, transparency and a step-change in the field.
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”
For this replication study, the authors classified papers on the topic of nanoparticle sensing and subsequently ranked them by number of citations. Based on this evaluation they selected a paper by Zhu and colleagues [14] entitled “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions” for their seminal replication study. To determine the reproducibility of the results from Zhu et al., the authors aim to establish the proportion of endosomal escape of the nanoparticles, and to examine the data in a biological context relevant to the application of the probe.
Beyond Replication
The authors plan to replicate the exact conditions reported in the materials and methods section of the selected paper such as nanoparticle probe synthesis of CdSe@C-TPEA nanoparticles, assessment of particle size, stability and reactivity and effect on cells (TEM, pH experiments, fluorescent responsivity to metal ions and cell viability). In addition, Said et al., plan to include further experimental characterisation to complement the existing study by Zhu and colleagues. They will incorporate additional controls and methodology to determine the intracellular location of nanoparticle probes in cells including: quantifying excess fluorescence in the culture medium, live cell imaging analysis, immunofluorescence with endosomal and lysosomal markers, and electron microscopy of cell sections. The authors will also include supplementary viability studies to assess the impact of the nanoparticles on HeLa cells as well as an additional biologically relevant cell line (for use in conjunction with the HeLa cells as per the original paper).
The Stage 1 manuscript underwent two rounds of thorough in-depth review. After considering the detailed responses to the reviewers' comments, the recommenders determined that the manuscript met the Stage 1 criteria and awarded in-principle acceptance (IPA).
The authors have thoughtfully considered their experimental approach to the replication study, whilst acknowledging any potential limitations. Given that conducting such a replication study is novel in the field of Nanotechnology and there is currently no ‘gold standard’ approach in doing so, the authors have showed thoughtful regard of statistical analysis and unbiased methodology where possible.
Based on current information, this study is the first use of preregistration via Peer Community in Registered Reports and the first formalised replication study in Nanotechnology for Biosciences. The outcomes of this of this study will be significant both scientifically and in the wider context in discussion of the scientific method.
URL to the preregistered Stage 1 protocol: https://osf.io/qbxpf
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA. List of eligible PCI RR-friendly Journals:
References
1. Howes, P. D., Chandrawati, R., & Stevens, M. M. (2014). Colloidal nanoparticles as advanced biological sensors. Science, 346(6205), 1247390. https://doi.org/10.1126/science.1247390
2. Liu, C. G., Han, Y. H., Kankala, R. K., Wang, S. B., & Chen, A. Z. (2020). Subcellular performance of nanoparticles in cancer therapy. International Journal of Nanomedicine, 675-704. https://doi.org/10.2147/IJN.S226186
3. Tang, W., Fan, W., Lau, J., Deng, L., Shen, Z., & Chen, X. (2019). Emerging blood–brain-barrier-crossing nanotechnology for brain cancer theranostics. Chemical Society Reviews, 48(11), 2967-3014. https://doi.org/10.1039/C8CS00805A
4. Yoon, Y. I., Pang, X., Jung, S., Zhang, G., Kong, M., Liu, G., & Chen, X. (2018). Smart gold nanoparticle-stabilized ultrasound microbubbles as cancer theranostics. Journal of Materials Chemistry B, 6(20), 3235-3239. https://doi.org/10.1039%2FC8TB00368H
5. Lin, H., Chen, Y., & Shi, J. (2018). Nanoparticle-triggered in situ catalytic chemical reactions for tumour-specific therapy. Chemical Society Reviews, 47(6), 1938-1958. https://doi.org/10.1039/C7CS00471K
6. Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021). Lipid nanoparticles for mRNA delivery. Nature Reviews Materials, 6(12), 1078-1094. https://doi.org/10.1038/s41578-021-00358-0
7. Mitchell, M. J., Billingsley, M. M., Haley, R. M., Wechsler, M. E., Peppas, N. A., & Langer, R. (2021). Engineering precision nanoparticles for drug delivery. Nature Reviews Drug Discovery, 20(2), 101-124. https://doi.org/10.1038/s41573-020-0090-8
8. Said, M., Gharib, M., Zrig, S., & Lévy, R. (2024). Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/qbxpf
9. Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M. A., Alkawareek, M. Y., Dreaden, E. C., ... & Mahmoudi, M. (2017). Cellular uptake of nanoparticles: Journey inside the cell. Chemical Society Reviews, 46(14), 4218-4244. https://doi.org/10.1039/C6CS00636A
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14. Zhu, A., Qu, Q., Shao, X., Kong, B., & Tian, Y. (2012). Carbon-dot-based dual-emission nanohybrid produces a ratiometric fluorescent sensor for in vivo imaging of cellular copper ions. Angewandte Chemie (International ed. in English), 51(29), 7185-7189. https://doi.org/10.1002/anie.201109089
| Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions” | Maha Said, Mustafa Gharib, Samia Zrig, Raphaël Lévy | <p>In hundreds of articles published over the past two decades, nanoparticles have been described as probes for sensing and imaging of a variety of intracellular cytosolic targets. However, nanoparticles generally enter cells by endocytosis with o... | | Life Sciences, Medical Sciences, Physical Sciences | Emily Linnane | 2023-11-29 19:14:03 | ||
30 May 2024
STAGE 1
The role of resource dynamics in the distribution of life cycles within a female human populationAn agent-based model of the role of resource dynamics and the environment in human female life cyclesRecommended by Claudio Tennie based on reviews by Cecilia Padilla-Iglesias and 1 anonymous reviewer
Among primates, the human female life cycle appears special. Aspects of these life cycles have been linked to the acquisition and distribution of resources and to environmental factors, as well as to individual differences across human females. Many questions remain regarding the causal roles that these (or also other) factors might have played in the evolution of human female life cycles – and also whether generalizing statements about these life cycles can adequately capture the wide range of the observed phenomena.
In the current study, Varas Enriquez et al. (2024) outline a plan for an agent-based model approach to study the factors that guide and channel variability in female life cycles in humans (within biological constraints), via the effects that their model will capture. The authors’ model has a particular eye towards the effects of resource dynamics (resource production and resource transfers) and environmental conditions – and their interplay. The results of this agent based model will be thoroughly analysed to better understand the evolution of the specific female human life cycle range.
The study plan was refined after one round of review, which led to input from two external reviewers and the recommender. The revised (second) version was judged to satisfy the Stage 1 criteria for in-principle acceptance.
URL to the preregistered Stage 1 protocol: https://osf.io/24c7z
Level of bias control achieved: Level 2. At least some data/evidence that will be used to answer the research question has been accessed and partially observed by the authors, but the authors certify that they have not yet observed the key variables within the data that will be used to answer the research question AND they have taken additional steps to maximise bias control and rigour (e.g. conservative statistical threshold; recruitment of a blinded analyst; robustness testing, multiverse/specification analysis, or other approach) List of eligible PCI RR-friendly journals: References
Varas Enríquez, P. J., Lukas, D., Colleran, H, Mulder, M. B., & Redhead, D. (2024) The role of resource dynamics in the distribution of life cycles within a female human population. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/24c7z
| The role of resource dynamics in the distribution of life cycles within a female human population | Pablo J. Varas Enríquez, Daniel Redhead, Monique Borgerhoff Mulder, Heidi Colleran, Dieter Lukas | <p>The evolution of the female human life cycle, which is characterised by having a reproductive career nested within juvenile and post-reproductive periods, has been linked to the surplus of adult resource production and downwards inter-generatio... | | Life Sciences, Medical Sciences, Social sciences | Claudio Tennie | 2023-11-13 15:45:52 |
