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IdTitle * Authors * Abstract * PictureThematic fields * RecommenderReviewers▲Submission date
24 Apr 2024
STAGE 1

Synaesthesia as a Model for Assessing Individual Differences in Visual Perception and Memory Performance

What can synaesthesia tell us about links between perception and memory?

Recommended by ORCID_LOGO based on reviews by Janina Neufeld, David Brang and Tessa van Leeuwen
What is the relationship between perception and memory? Although these topics are typically investigated separately, there is evidence that these cognitive processes may be related: for example, individuals with synaesthesia may experience both enhancements in visual acuity and visual memory; and individuals with amnesia may also show deficits in visual perceptual processing. However, comprehensive evidence for the relationship between perception and different forms of memory (both short-term and long-term) is currently lacking.
 
In this Stage 1 manuscript, Whelan et al. (2024) seek to elucidate this relationship by investigating individual differences in perception and memory in a general population sample (i.e., in synaesthetes, non-synaesthetic relatives, and controls). There are two accounts that may explain enhanced perception and memory in synaesthesia: a ‘dual-coding’ account, which suggests that the extra perceptual information often experienced in synaesthesia (e.g., seeing colors for different letters of the alphabet) may contribute to encoding richer information in sensory memory; and an ‘enhanced processing’ account, which posits that enhanced perception and memory in synaesthesia may be due to genetic or environmental factors not directly related to synaesthetic experiences. In the former case, synaesthetes should perform more similarly to each other than to their non-synaesthetic relatives; in the latter case, non-synaesthetic relatives of synaesthetes should show similar perceptual and memory benefits. The current study should therefore find evidence in favor of one of these accounts over the other. In addition to this, the authors will generate multidimensional cognitive profiles of synaesthetes and their relatives, compared to non-synaethetes, including perception, memory, mental imagery and cognitive styles. 
 
The Stage 1 submission was evaluated by the recommender and two expert reviewers. Following revisions, the recommender judged that the manuscript met the Stage 1 criteria and awarded in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/6h8dx (under temporary private embargo)
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly journals:
 
 
References
 
1. Whelan, E., Sachdeva, C., Ovalle-Fresa, R., Rothen R., & Ward, J. (2024). Synaesthesia as a Model for Assessing Individual Differences in Visual Perception and Memory Performance. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/6h8dx
Synaesthesia as a Model for Assessing Individual Differences in Visual Perception and Memory PerformanceEmily Whelan, Chhavi Sachdeva, Rebecca Ovalle-Fresa, Nicolas Rothen and Jamie Ward<p>In this study, the cognitive profile of synaesthesia (a perceptual condition in which primary experiences, such as perceiving digits or words, elicit extra secondary sensations) is used as a model system to assess visual perceptual abilities an...Social sciencesReshanne Reeder2023-11-07 13:02:39 View
30 May 2024
STAGE 1
article picture

The role of resource dynamics in the distribution of life cycles within a female human population

An agent-based model of the role of resource dynamics and the environment in human female life cycles

Recommended by ORCID_LOGO based on reviews by Cecilia Padilla-Iglesias and 1 anonymous reviewer
Among primates, the human female life cycle appears special. Aspects of these life cycles have been linked to the acquisition and distribution of resources and to environmental factors, as well as to individual differences across human females. Many questions remain regarding the causal roles that these (or also other) factors might have played in the evolution of human female life cycles – and also whether generalizing statements about these life cycles can adequately capture the wide range of the observed phenomena.
 
In the current study, Varas Enriquez et al. (2024) outline a plan for an agent-based model approach to study the factors that guide and channel variability in female life cycles in humans (within biological constraints), via the effects that their model will capture. The authors’ model has a particular eye towards the effects of resource dynamics (resource production and resource transfers) and environmental conditions – and their interplay. The results of this agent based model will be thoroughly analysed to better understand the evolution of the specific female human life cycle range.
 
The study plan was refined after one round of review, which led to input from two external reviewers and the recommender. The revised (second) version was judged to satisfy the Stage 1 criteria for in-principle acceptance.
 
URL to the preregistered Stage 1 protocol: https://osf.io/24c7z
 
Level of bias control achieved: Level 2. At least some data/evidence that will be used to answer the research question has been accessed and partially observed by the authors, but the authors certify that they have not yet observed the key variables within the data that will be used to answer the research question AND they have taken additional steps to maximise bias control and rigour (e.g. conservative statistical threshold; recruitment of a blinded analyst; robustness testing, multiverse/specification analysis, or other approach)
 
List of eligible PCI RR-friendly journals:
 
 
References
 
Varas Enríquez, P. J., Lukas, D., Colleran, H, Mulder, M. B., & Redhead, D. (2024) The role of resource dynamics in the distribution of life cycles within a female human population. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/24c7z
The role of resource dynamics in the distribution of life cycles within a female human populationPablo J. Varas Enríquez, Daniel Redhead, Monique Borgerhoff Mulder, Heidi Colleran, Dieter Lukas<p>The evolution of the female human life cycle, which is characterised by having a reproductive career nested within juvenile and post-reproductive periods, has been linked to the surplus of adult resource production and downwards inter-generatio...Life Sciences, Medical Sciences, Social sciencesClaudio Tennie2023-11-13 15:45:52 View
26 Feb 2024
STAGE 1

Lure of choice revisited: Replication and extensions Registered Report of Bown et al. (2003)

Replicating the "lure of choice" phenomenon

Recommended by ORCID_LOGO based on reviews by Hu Chuan-Peng and Gakuto Chiba
The "lure of choice" refers to the idea that we prefer to preserve the option to choose even when the choice is not helpful. In a classic study cited hundred of times, Bown et al. (2003) reported evidence for the lure of choice from a series of studies involving choices between competing options of night clubs, bank savings accounts, casino spinners, and the Monty Hall door choice paradigm. In all cases, participants tended to prefer to choose an option when paired with a "lure", even when that lure was objectively inferior (e.g., same probability of winning but lower payoff).
 
The lure of choice phenomenon applies to a variety of real-life situations many of us often face in our daily lives, and Bown et al.’s findings have influenced the way organizations present choices to prospective users. Despite their theoretical and practical impact, Bown et al.'s findings have not previously been directly replicated, even as the importance of replication studies has become increasingly acknowledged (Nosek et al., 2022).
 
Here, Chan & Feldman (2024) outline a close replication of Bown et al. (2003) that will replicate and extend their original design. By unifying Bown et al.'s multiple studies into a single paradigm with which they will collect data from approximately 1,000 online participants via Prolific, they will have substantially greater statistical power than the original study to detect the predicted effects. They will follow LeBel et al.’s (2019) criteria for evaluating replicability, such that it will be considered a successful replication depending on how many of the 4 scenarios show a signal in the same direction as Bown et al.’s original results (at least 3 out of 4 scenarios = successful replication; no scenarios = failed replication; 1 or 2 scenarios = mixed results replication). They have also added additional controls including a neutral baseline choice without a lure, further ensuring the the validity and interpretability of their eventual findings.
 
One of the goals in creating Peer Community In Registered Reports (PCI RR) was to increase the availability of publishing venues for replication studies, and so PCI RR is well-suited to the proposed replication. Feldman’s lab has also pioneered the use of PCI RR for direct replications of previous studies (e.g., Zhu & Feldman, 2023), and the current submission uses an open-access template he developed (Feldman, 2023). This experience combined with PCI RR’s efficient scheduled review model meant that the current full Stage 1 protocol was able to go from initial submission, receive detailed peer review by two experts, and receive in-principle acceptance (IPA) for the revised submission, all in less than one month.
 
URL to the preregistered Stage 1 protocol: https://osf.io/8ug9m
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly journals:
 
 
References
 
Bown, N. J., Read, D. & Summers, B. (2003). The lure of choice. Journal of Behavioral Decision Making, 16(4), 297–308. https://doi.org/10.1002/bdm.447
 
Chan, A. N. Y. & Feldman, G. (2024). The lure of choice revisited: Replication and extensions Registered Report of Bown et al. (2003) [Stage 1]. In principle acceptance of Version 2 by Peer Community In Registered Reports. https://osf.io/8ug9m
 
Feldman, G. (2023). Registered Report Stage 1 manuscript template. https://doi.org/10.17605/OSF.IO/YQXTP
 
LeBel, E. P., Vanpaemel, W., Cheung, I. & Campbell, L. (2019). A brief guide to evaluate replications. Meta-Psychology, 3. https://doi.org/10.15626/MP.2018.843
 
Nosek, B. A., Hardwicke, T. E., Moshontz, H., Allard, A., Corker, K. S., Dreber, A., ... & Vazire, S. (2022). Replicability, robustness, and reproducibility in psychological science. Annual Review of Psychology, 73(1), 719-748. https://doi.org/10.1146/annurev-psych-020821-114157
 
Zhu, M. & Feldman, G. (2023). Revisiting the links between numeracy and decision making: Replication Registered Report of Peters et al. (2006) with an extension examining confidence. Collabra: Psychology, 9(1). https://doi.org/10.1525/collabra.77608
Lure of choice revisited: Replication and extensions Registered Report of Bown et al. (2003)Nga Yi (Angela) Chan, Gilad Feldman<p>[IMPORTANT: Abstract, method, and results were written using a randomised dataset produced by Qualtrics to simulate what these sections will look like after data collection. These will be updated following the data collection. For the purpose o...Social sciencesPatrick Savage2023-11-15 00:40:47 View
09 Sep 2024
STAGE 1

How Effortful is Boredom? Studying Self-Control Demands Through Pupillometry

Unmasking the effort of boredom: A deeper look into self-control dynamics

Recommended by ORCID_LOGO based on reviews by Jonas Dora, Julia Englert, Erik Bijleveld and 1 anonymous reviewer
Effective effort regulation is central for self-control and has been a focus of psychological research for many decades. It is well established that challenging cognitive tasks, which impose self-control demands due to their complexity, are perceived as more effortful and require greater physiological activation than simpler tasks. However, the experience and allocation of effort during a task, whether easy or difficult, likely depends on more than these inherent self-control demands. Another key factor is boredom, which can arise during task execution. Yet, the role of boredom and the interplay with perceived task difficulty over time remains underexamined.
 
Based on these considerations, Radtke et al. (2024) aim to examine how boredom and task difficulty influence both subjective and objective measures of effort. They propose that subjective cognitive effort consists of both boredom-related and difficulty-related components, hypothesizing that both factors together more accurately predict objective effort allocation than task difficulty alone. The proposed study will use a within-subject design, in which participants perform two versions of the Stroop task: an easy version with only congruent trials and a hard version. Thought probes during the task will track subjective boredom, fatigue, perceived difficulty, and effort. Phasic and stimulus-evoked pupil dilation will serve as an objective index of cognitive effort, while a subsequent flanker task will assess effects on secondary task performance. 
 
The authors predict that the easy Stroop version will result in lower perceived difficulty but higher boredom, as well as fewer errors and faster reaction times compared to the hard version. Over time, boredom and perceived effort due to boredom are expected to increase, while perceived task difficulty and effort attributed to difficulty are anticipated to decrease. Linear Mixed Models will be employed to determine whether phasic and stimulus-evoked pupil dilation can be predicted by effort attributed to boredom, difficulty, or both. This innovative approach combines objective and subjective measures and promises to deepen our understanding of the cognitive demands posed by boredom, with significant implications for research on effort regulation and motivation. 
 
The Stage 1 manuscript was evaluated by four expert reviewers in two rounds of in-depth review. Following responses from the authors, the recommender determined that Stage 1 criteria were met and awarded in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/38qz4

Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly Journals:
 
 
References

Radtke, V., Wolff, W., & Martarelli, C. S. (2024). How effortful is boredom? Studying self-control demands through pupillometry. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/38qz4
How Effortful is Boredom? Studying Self-Control Demands Through PupillometryVanessa C. Radtke, Wanja Wolff, Corinna S. Martarelli<p>Self-control is essential for managing our actions, yet its exertion is perceived as effortful. Performing a task may require effort not only because of its inherent difficulty but also due to its potential for inducing boredom, as boredom has ...Humanities, Social sciencesThomas Meyer2023-11-21 17:56:13 View
28 Feb 2024
STAGE 2
(Go to stage 1)

Genetically-modified animals as models of neurodevelopmental conditions: a review of systematic review reporting quality

Evidence for mixed quality of systematic reviews in preclinical animal studies of neurodevelopmental conditions

Recommended by ORCID_LOGO based on reviews by Marietta Papadatou-Pastou
Single gene alterations have been estimated to account for nearly half of neurodevelopmental conditions (NDCs), providing a crucial opportunity for animal models to understand the underlying mechanisms, causes and potential treatments. The use of systematic reviews (SRs) can, in principle, provide a powerful means to synthesise this evidence-base; however, the reporting quality of previous SRs in preclinical animal research has been found lacking (Hunniford et al., 2021). In the current study, Wilson et al. (2023) will undertook a review of systematic reviews to assess the characteristics and reporting quality of SRs that, in turn, synthesise research in genetically-modified animals to model NDCs. In particular, the authors extracted key features of reviews (including, among others, the aim and primary research questions, relevant animal model, and number of studies in the SR), in addition to quality indicators such as risk of bias and completeness of reporting. In doing so, the authors aimed to enhance guidance on the conduct and reporting of systematic reviews in this area.
 
Of twelve publications that met the preregistered search criteria, the completeness and quality of reporting was variable. Among the better reported characteristics were search strategies (9 of 12 articles), reporting of funding sources (10 of 12 articles) and use of animal data (11 of 12 articles). In contrast, only two articles reported whether the study protocol was preregistered, only three articles reported methods for assessing risk of bias, and just one included methods to analyse publication bias. In addition, the authors identified 19 review registrations via PROSPERO, most of which remained unpublished after their anticipated end dates. Overall, the results highlight the importance of adherence to reporting guidelines for increasing the transparency and reproducibility of SRs in this field.
 
The Stage 2 manuscript was evaluated over one round of in-depth review. Based on detailed responses by the authors, the recommender judged that the manuscript met the Stage 2 criteria and awarded a positive recommendation.
 
URL to the preregistered Stage 1 protocol: https://osf.io/952qk
 
Level of bias control achieved: Level 4. At least some of the data/evidence that was used to answer the research question already existed prior to IPA and was accessible in principle to the authors, but the authors certify that they did not access any part of that data/evidence prior to IPA.
 
List of eligible PCI RR-friendly journals:
 
References
 
1. Hunniford V. T., Montroy J., Fergusson D. A., Avey M. T., Wever K. E., McCann S. K., Foster M., Fox G., Lafreniere M., Ghaly M., Mannell S., Godwinska K., Gentles A., Selim S., MacNeil J., Sikora L., Sena E. S., Page M. J., Macleod M., Moher D., & Lalu M. M. (2021). Epidemiology and reporting characteristics of preclinical systematic reviews. PLOS Biology, 19:e3001177. https://doi.org/10.1371/journal.pbio.3001177
 
2. Wilson, E., Currie, G., Macleod, M., Kind, P. & Sena, E. S. (2023). Genetically-modified animals as models of neurodevelopmental conditions: a review of systematic review reporting quality [Stage 2]. Acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/s5xd4
Genetically-modified animals as models of neurodevelopmental conditions: a review of systematic review reporting qualityEmma Wilson, Gillian Currie, Malcolm Macleod, Peter Kind, and Emily S Sena<p><strong>Objective</strong><br>Using genetically-modified animals to model neurodevelopmental conditions (NDCs) helps better our understanding of biology underlying these conditions. Animal research has unique characteristics not shared with cli...Medical SciencesChris Chambers2023-11-22 10:26:44 View
21 Apr 2024
STAGE 1

Mechanisms of secularization: Testing between three causal pathways

Understanding links between secularization, rationalisation and insecurity

Recommended by ORCID_LOGO based on reviews by Chris Chambers and 1 anonymous reviewer
What relationship can be expected between secularization, rationalisation and insecurity? While some authors argue that rationalisation reduces the willingness to belong to religious groups, others have suggested that insecurity increases this need to belong to religious groups.
 
In the current study, Lang and Chvaja (2024) will adjudicate between these two possibilities using an economics game in participants from two countries: US and Poland. The central question posed by the authors is whether cooperative insecurity increases the probability of joining a religious normative group. They will test the relationship between an environment (secure and insecure) and institution (which related to the norm context: religious and secular) on the probability of choosing the normative group in an experimental setting. Therefore, the study will be a quantitative analysis.
 
The authors included an adequate power analysis, alternatives for non-supported hypotheses, and filtering to ensure a high quality of data collection. They also undertook a pilot study to ensure the quality of the procedure and sensitivity of the analyses.
 
The Stage 1 manuscript was evaluated over one round of in-depth review. Based on ​detailed responses to reviewers’ and the recommender’s comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance.​​​
 
URL to the preregistered Stage 1 protocol: https://osf.io/yzgek
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly journals:
 
 
References
 
1.Lang, M. & Chvaja, R. (2024). Mechanisms of secularization: Testing between the rationalization and existential insecurity theories. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/yzgek
Mechanisms of secularization: Testing between three causal pathwaysMartin Lang, Radim Chvaja<p>The study tests two competing explanations of the secularization process related to rationalizing worldviews and decreasing existential insecurity. While the former explanation argues that people are unwilling to join religious groups because o...Social sciencesAdrien Fillon2023-11-22 11:17:30 View
27 Mar 2024
STAGE 1

Revisiting the signal value of emotion in altruistic behavior: Replication and extensions Registered Report of Barasch et al. (2014) Studies 3 and 6

Understanding how motives and emotions driving prosocial actions impact the moral assessment of good doers

Recommended by ORCID_LOGO based on reviews by Thibaut Arpinon and Angela Sutan
Pro-social actions are often driven by emotional factors. For instance, emotions have been shown to play a decisive role in the way we judge the fairness of a situation (affect-as-information theory: e.g., Clore et al., 2001; Storbeck and Clore, 2008), and, thus, how we make decisions. Specific emotions like anger have also been shown to stimulate the motivation to fight injustice (Lerner et al., 2015). At the individual level, people might undertake altruistic actions as a way to relieve themselves from these negative emotions (what Cialdini (1991) calls ‘reflexive distress’) but also because these actions are expected by the social norms (‘normative distress’). Indeed, pro-social actions are usually taken in social contexts, and the perception of one’s behavior by third parties might hinder or facilitate the adoption of pro-social behaviors. Understanding the determinants of the perception of altruistic behaviors is thus a key research question to support pro-social actions in collective settings.
 
In the current study, Woo and Feldman (2024) aim to replicate the seminal work of Barasch et al. (2014), who showed that third parties hold more favorable views of agents undertaking pro-social actions when the latter are motivated by emotions. More precisely, the authors aim to replicate two studies of the original work by conducting a well-powered online experiment (US participants, Prolific, N=1,164). First, they will investigate whether donors who exhibit higher distress regarding the suffering of others are perceived as more moral and authentically concerned for others. Second, they will analyze whether individuals who expect material or reputational benefits from their altruistic deeds are perceived by third parties as less moral than those who act for emotional reasons. In addition to these two replication objectives, the authors propose extensions with pre-registered hypotheses that are inspired by Study 2 from the original work. They seek to investigate whether people are seen as more other-focused when they undertake a prosocial action (donation) and under different expected rewards (material, reputational, emotional benefits).
 
The Stage 1 manuscript was evaluated by two external reviewers and the recommender. Based on detailed responses to the reviewers' and the recommender’s comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/d5bmp

Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly journals:
 

References

1. Barasch, A., Levine, E. E., Berman, J. Z., & Small, D. A. (2014). Selfish or selfless? On the signal value of emotion in altruistic behavior. Journal of Personality and Social Psychology, 107, 393-413. https://doi.org/10.1037/a0037207
 
2. Cialdini, R. B. (1991). Altruism or egoism? That is (still) the question. Psychological Inquiry, 2, 124-126. https://psycnet.apa.org/doi/10.1207/s15327965pli0202_3
 
3. Clore, G. L., Gasper, K., Garvin, E., & Forgas, J. P. (2001). Handbook of Affect and Social Cognition.
 
4. Lerner, J. S., Li, Y., Valdesolo, P., & Kassam, K. S. (2015). Emotion and decision making. Annual Review of Psychology, 66, 799-823. https://doi.org/10.1146/annurev-psych-010213-115043
 
5. Storbeck, J., & Clore, G. L. (2008). Affective arousal as information: How affective arousal influences judgments, learning, and memory. Social and Personality Psychology Compass, 2, 1824-1843. https://doi.org/10.1111%2Fj.1751-9004.2008.00138.x
 
6. Woo, T. L. & Feldman, G. (2024). Revisiting the signal value of emotion in altruistic behavior: Replication and extensions Registered Report of Barasch et al. (2014) Studies 3 and 6. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/d5bmp
Revisiting the signal value of emotion in altruistic behavior: Replication and extensions Registered Report of Barasch et al. (2014) Studies 3 and 6Tse Lyn (Rachael) Woo; Gilad Feldman<p>[IMPORTANT: Abstract, method, and results were written using a randomized dataset produced by Qualtrics to simulate what these sections will look like after data collection. These will be updated following the data collection. For the purpose o...Social sciencesRomain Espinosa2023-11-23 05:22:23 View
31 May 2024
STAGE 1

Representativeness heuristic in intuitive predictions: Replication Registered Report of problems reviewed in Kahneman and Tversky (1973)

The Representativeness Heuristic Revisited: Registered Replication Report of Kahneman and Tversky (1973)

Recommended by ORCID_LOGO based on reviews by Peter Anthony White, Regis Kakinohana and Naseem Dillman-Hasso
Revisiting a true classic, this registered replication report addresses Kahneman and Tversky’s (1973) introduction of the representativeness heuristic. The heuristic refers to deviations of judgments from normative evaluations of the evidence when the stimulus fits to a prototype. For instance, when an individual is described by features stereotypically associated with a certain target group (e.g., a person who attends dance training several times a week and has a passion for singing and performing), likelihood judgments that the individual belongs to a target group (K-Pop artists) compared to a non-target group (e.g., accountants) are inflated. 

The impact of the original research on the field is clearly immense and long-lasting. All the better that a systematic replication attempt is being undertaken in this registered report, which addresses studies 1 through 7 of Kahneman and Tversky’s classic 1973 paper. Chan and Feldman (2024) propose a well-powered online study, in which all studies from the original article are presented to participants within-subjects. The materials are carefully constructed and closely documented in the accompanying OSF project, where in-depth information on planned data analyses is supported with a simulated dataset.  

The Stage 1 manuscript was evaluated over three rounds of in-depth review. Based on detailed responses to the reviewers' comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/er2cq
 
Level of bias control achieved: Level 6. Data collection commenced during the later part of Stage 1 peer review; however, since no substantive changes to the design were made after this point, the risk of bias due to prior data observation remains zero and the manuscript therefore qualifies for Level 6.
 
List of eligible PCI RR-friendly journals:
 
 
References

1. Chan, H. C. & Feldman, G. (2024). Representativeness heuristic in intuitive predictions: Replication Registered Report of problems reviewed in Kahneman and Tversky (1973). In principle acceptance of Version 5 by Peer Community in Registered Reports. https://osf.io/er2cq
 
2. Kahneman, D., & Tversky, A. (1973). On the psychology of prediction. Psychological Review, 80(4), 237–251. https://doi.org/10.1037/h0034747
 
Representativeness heuristic in intuitive predictions: Replication Registered Report of problems reviewed in Kahneman and Tversky (1973)Hong Ching (Bruce) Chan, Gilad Feldman<p>[IMPORTANT: Abstract, method, and results were written using a randomized dataset produced by Qualtrics to simulate what these sections will look like after data collection. These will be updated following the data collection. For the purpose o...Social sciencesRima-Maria Rahal2023-11-29 15:19:07 View
22 Jul 2024
STAGE 1
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Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”

Replicating, Revising and Reforming: Unpicking the Apparent Nanoparticle Endosomal Escape Paradox

Recommended by and ORCID_LOGO based on reviews by Cecilia Menard-Moyon and Zeljka Krpetic
Context
 
Over the past decade there has been an exponential increase in the number of research papers utlising nanoparticles for biological applications such as intracellular sensing [1, 2], theranostics [3-5] and more recently drug delivery and precision medicine [6, 7]. Despite the success stories, there is a disconnect regarding current dogma on issues such as nanoparticle uptake and trafficking, nanoparticle delivery via the enhanced permeability and retention (EPR) effect, and endosomal escape. Critical re-evaluation of these concepts both conceptually and experimentally is needed for continued advancement in the field.
 
For this preregistration, Said et al. (2024) focus on nanoparticle intracellular trafficking, specifically endosomal escape [8]. The current consensus in the literature is that nanoparticles enter cells via endocytosis [9, 10] but reportedly just 1-2% of nanoparticles/ nanoparticle probes escape endosomes and enter the cytoplasm [11-13]. There is therefore an apparent paradox over how sensing nanoparticles can detect their intended targets in the cytoplasm if they are trapped within the cell endosomes. To address this fundamental issue of nanoparticle endosomal escape, Lévy and coworkers are carrying out replication studies to thoroughly and transparently replicate the most influential papers in the field of nanoparticle sensing. The aim of these replication studies is twofold: to establish a robust methodology to study endosomal escape of nanoparticles, and to encourage discussions, transparency and a step-change in the field.  
 
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”
 
For this replication study, the authors classified papers on the topic of nanoparticle sensing and subsequently ranked them by number of citations.  Based on this evaluation they selected a paper by Zhu and colleagues [14] entitled “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions” for their seminal replication study.  To determine the reproducibility of the results from Zhu et al., the authors aim to establish the proportion of endosomal escape of the nanoparticles, and to examine the data in a biological context relevant to the application of the probe.
 
Beyond Replication
 
The authors plan to replicate the exact conditions reported in the materials and methods section of the selected paper such as nanoparticle probe synthesis of CdSe@C-TPEA nanoparticles, assessment of particle size, stability and reactivity and effect on cells (TEM, pH experiments, fluorescent responsivity to metal ions and cell viability). In addition, Said et al., plan to include further experimental characterisation to complement the existing study by Zhu and colleagues. They will incorporate additional controls and methodology to determine the intracellular location of nanoparticle probes in cells including: quantifying excess fluorescence in the culture medium, live cell imaging analysis, immunofluorescence with endosomal and lysosomal markers, and electron microscopy of cell sections. The authors will also include supplementary viability studies to assess the impact of the nanoparticles on HeLa cells as well as an additional biologically relevant cell line (for use in conjunction with the HeLa cells as per the original paper).
 
The Stage 1 manuscript underwent two rounds of thorough in-depth review. After considering the detailed responses to the reviewers' comments, the recommenders  determined that the manuscript met the Stage 1 criteria and awarded in-principle acceptance (IPA).  
 
The authors have thoughtfully considered their experimental approach to the replication study, whilst acknowledging any potential limitations. Given that conducting such a replication study is novel in the field of Nanotechnology and there is currently no ‘gold standard’ approach in doing so, the authors have showed thoughtful regard of statistical analysis and unbiased methodology where possible.
 
Based on current information, this study is the first use of preregistration via Peer Community in Registered Reports and the first formalised replication study in Nanotechnology for Biosciences. The outcomes of this of this study will be significant both scientifically and in the wider context in discussion of the scientific method.
 
URL to the preregistered Stage 1 protocol: https://osf.io/qbxpf
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly Journals:
 
References
 
1. Howes, P. D., Chandrawati, R., & Stevens, M. M. (2014). Colloidal nanoparticles as advanced biological sensors. Science, 346(6205), 1247390. https://doi.org/10.1126/science.1247390
 
2. Liu, C. G., Han, Y. H., Kankala, R. K., Wang, S. B., & Chen, A. Z. (2020). Subcellular performance of nanoparticles in cancer therapy. International Journal of Nanomedicine, 675-704. https://doi.org/10.2147/IJN.S226186
 
3. Tang, W., Fan, W., Lau, J., Deng, L., Shen, Z., & Chen, X. (2019). Emerging blood–brain-barrier-crossing nanotechnology for brain cancer theranostics. Chemical Society Reviews, 48(11), 2967-3014. https://doi.org/10.1039/C8CS00805A
 
4. Yoon, Y. I., Pang, X., Jung, S., Zhang, G., Kong, M., Liu, G., & Chen, X. (2018). Smart gold nanoparticle-stabilized ultrasound microbubbles as cancer theranostics. Journal of Materials Chemistry B, 6(20), 3235-3239. https://doi.org/10.1039%2FC8TB00368H
 
5. Lin, H., Chen, Y., & Shi, J. (2018). Nanoparticle-triggered in situ catalytic chemical reactions for tumour-specific therapy. Chemical Society Reviews, 47(6), 1938-1958. https://doi.org/10.1039/C7CS00471K
 
6. Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021). Lipid nanoparticles for mRNA delivery. Nature Reviews Materials, 6(12), 1078-1094. https://doi.org/10.1038/s41578-021-00358-0
 
7. Mitchell, M. J., Billingsley, M. M., Haley, R. M., Wechsler, M. E., Peppas, N. A., & Langer, R. (2021). Engineering precision nanoparticles for drug delivery. Nature Reviews Drug Discovery, 20(2), 101-124. https://doi.org/10.1038/s41573-020-0090-8
 
8. Said, M., Gharib, M., Zrig, S., & Lévy, R. (2024). Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/qbxpf
 
9. Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M. A., Alkawareek, M. Y., Dreaden, E. C., ... & Mahmoudi, M. (2017). Cellular uptake of nanoparticles: Journey inside the cell. Chemical Society Reviews, 46(14), 4218-4244. https://doi.org/10.1039/C6CS00636A
 
10. de Almeida, M. S., Susnik, E., Drasler, B., Taladriz-Blanco, P., Petri-Fink, A., & Rothen-Rutishauser, B. (2021). Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine. Chemical society reviews, 50(9), 5397-5434. https://doi.org/10.1039/D0CS01127D
 
11. Smith, S. A., Selby, L. I., Johnston, A. P., & Such, G. K. (2018). The endosomal escape of nanoparticles: toward more efficient cellular delivery. Bioconjugate Chemistry, 30(2), 263-272. http://dx.doi.org/10.1021/acs.bioconjchem.8b00732
 
12. Cupic, K. I., Rennick, J. J., Johnston, A. P., & Such, G. K. (2019). Controlling endosomal escape using nanoparticle composition: current progress and future perspectives. Nanomedicine, 14(2), 215-223. https://doi.org/10.2217/nnm-2018-0326
 
13. Wang, Y., & Huang, L. (2013). A window onto siRNA delivery. Nature Biotechnology, 31(7), 611-612. https://doi.org/10.1038/nbt.2634
 
14. Zhu, A., Qu, Q., Shao, X., Kong, B., & Tian, Y. (2012). Carbon-dot-based dual-emission nanohybrid produces a ratiometric fluorescent sensor for in vivo imaging of cellular copper ions. Angewandte Chemie (International ed. in English), 51(29), 7185-7189. https://doi.org/10.1002/anie.201109089
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”Maha Said, Mustafa Gharib, Samia Zrig, Raphaël Lévy <p>In hundreds of articles published over the past two decades, nanoparticles have been described as probes for sensing and imaging of a variety of intracellular cytosolic targets. However, nanoparticles generally enter cells by endocytosis with o...Life Sciences, Medical Sciences, Physical SciencesEmily Linnane2023-11-29 19:14:03 View
13 Sep 2024
STAGE 1

Personality traits predict perception of pandemic risk and compliance with infection control measures

Associations between personality traits, compliance and risk perception in Norwegian adults during COVID-19

Recommended by based on reviews by Xiaowen Xu and 1 anonymous reviewer
This study aims to examine the associations between personality traits, using the big-5 personality model (Costa & McCrae, 1992), and compliance with pandemic measures (e.g. handwashing), and perceived risk of the pandemic to the individual, in a representative sample of the adult Norwegian population.
 
Several previous studies have examined associations between personality and adherence to medical advice using measures administered at the same point in time the during COVID-19 pandemic (e.g. Götz et al., 2021), however the dataset the current investigators plan to use assessed personality one year prior to the pandemic (May/June 2019) and the risk and compliance measures four months into the pandemic (August/September 2020). 
 
Here, Sætrevik et al. (2024) make a number of hypotheses, including that conscientiousness, agreeableness, openness and neuroticism will be positively associated with compliance; extraversion will be negatively associated with compliance; extraversion and openness will be negatively associated with perceived risk; and finally, neuroticism will be positively associated with perceived risk. They plan to test their hypotheses using linear regression analyses.
 
Importantly, given the topic, the data for this study already exists and some response distributions of the compliance and risk measures have been examined. However, the authors have yet to link the data on personality to the data on compliance and risk, which is the focus of their hypotheses.  
 
The findings from this study will help us to understand the personality characteristics associated with infection control measures and individual risk perceptions of viral illnesses during a pandemic. 
 
The Stage 1 manuscript was evaluated by two expert reviewers in two rounds of in-depth review. Following responses from the authors, the recommender determined that Stage 1 criteria were met and awarded in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/p5sjb
 
Level of bias control achieved: Level 1. At least some of the data/evidence that will be used to the answer the research question has been accessed and observed by the authors, including key variables, but the authors certify that they have not yet performed any of their preregistered analyses, and in addition they have taken stringent steps to reduce the risk of bias.
 
List of eligible PCI RR-friendly journals:
 
 
References

1. Costa, P. T., and McCrae, R. R. (1992). The five-factor model of personality and its relevance to personality disorders. Journal of Personality Disorders, 6, 343–359. https://doi.org/10.1521/pedi.1992.6.4.343
 
2. Götz, F. M., Gvirtz, A., Galinsky, A. D., and Jachimowicz, J. M. (2021). How personality and policy predict pandemic behavior: Understanding sheltering-in-place in 55 countries at the onset of COVID-19. The American psychologist, 76, 39–49. https://doi.org/10.1037/amp0000740
 
3. Sætrevik, B., Erevik, E. K., and Bjørkheim, S. B. (2024). Personality traits predict perception of pandemic risk and compliance with infection control measures. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/p5sjb
Personality traits predict perception of pandemic risk and compliance with infection control measuresBjørn Sætrevik, Eilin K. Erevik, Sebastian B. Bjørkheim<p>Personality traits influence our outlook and choices in life, and may also influence how we evaluate and respond to an extreme event such as the COVID-19 pandemic in 2020. Here we combined big-5 personality measures from a large nationally repr...Social sciencesAndrew Jones2023-11-30 23:17:12 View