Submit a report

Announcements

We are recruiting recommenders (editors) from all research fields!

Your feedback matters! If you have authored or reviewed a Registered Report at Peer Community in Registered Reports, then please take 5 minutes to leave anonymous feedback about your experience, and view community ratings.

Latest recommendations

IdTitle * Authors * Abstract * PictureThematic fields * RecommenderReviewersSubmission date
09 Sep 2024
STAGE 1

The effects of isolated game elements on adherence rates in food-based response inhibition training

Using gamification to improve food response inhibition training

Recommended by ORCID_LOGO based on reviews by Miguel Vadillo and 2 anonymous reviewers
A poor diet has severe detrimental health effects, and attempts to reduce caloric intake often prove unsuccessful. Unhealthy foods, high in fat, sugar, and salt tend to be highly appetitive, and can undermine individuals’ ability to refrain themselves from consuming them. Computerized cognitive retraining techniques have shown promise in curbing the intake of unhealthy foods and promoting weight loss. However, in real-world scenarios, adherence to such retraining programs can be suboptimal, potentially diminishing their effectiveness.
 
In the present study, Maclellan et al. (2023) aim to investigate whether the incorporation of gamified elements, transforming the cognitive retraining task into a game-like experience, can enhance adherence and overall intervention effectiveness by boosting engagement and motivation. These findings could deepen our understanding of the mechanisms through which cognitive retraining mitigates caloric intake and pave the way for more compelling and successful interventions.
 
The Stage 1 manuscript was evaluated over one round of review and revision. Based on detailed evaluations by two expert reviewers, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/jspf3 (under temporary private embargo)

Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly journals:
 

References
 
1. MacLellan, A., Pennington, C., Lawrence, N., Westwood, S., Jones, A., & Button, K. (2023). The effects of isolated game elements on adherence rates in food-based response inhibition training. In principle acceptance of Version 1.6 by Peer Community in Registered Reports. https://osf.io/jspf3

The effects of isolated game elements on adherence rates in food-based response inhibition trainingAlexander MacLellan, Charlotte Pennington, Natalia Lawrence, Samuel Westwood, Andrew Jones, Katherine Button<p>Introduction: Poor diet, and the consumption of foods high in fat, sugar and salt are common causes of premature mortality and health conditions. Computerised response inhibition training has been proposed to devalue these foods by learning to ...Social sciencesMateo Leganes-Fonteneau2023-07-14 16:24:03 View
09 Sep 2024
STAGE 1

The origin of symbolic numerical knowledge in early development – an fNIRS Registered Report

An fNIRS test of the neural correlates of the Cardinality Principle in typically-developing children

Recommended by ORCID_LOGO based on reviews by Ed Hubbard and 1 anonymous reviewer
The cardinality principle (CP) is a key concept in numerical cognition, which children typically acquire by the age of five. This is the understanding that each number in a counted sequence refers to a specific set of items, and that the final number represents the total size of the set. Here, Ivanova and colleagues (2024) propose an experiment to study the changes in neural activity that accompany the acquisition of this concept, focusing on activity within the parietal lobes as measured by functional near-infrared spectroscopy (fNIRS).
 
Typically-developing children aged from 2 years 9 months to 4 years 9 months will be assessed for their ability to give a specific number of balls from a pile: those who can give five or more correctly will be classified as CP-knowers, and those who can only give lower set sizes will be classified as subset-knowers. All children will then perform an auditory number word adaptation task while undergoing fNIRS. The adaptation task involves hearing the number word ‘two’ repeated, interspersed with deviant number words (‘four’ or ‘eight’) or a non-number word (‘rin’). The experimental hypotheses are that left parietal activations and bilateral parietal functional connectivity will be differentially greater for number than non-number deviants amongst CP-knowers than amongst subset-knowers. Each hypothesis will be tested by sequential Bayes factor analysis, with a minimum of 25 and a maximum of 46 participants per group, providing high sensitivity to detect a smallest effect size of d = .35. This study aims to provide insights into the neural underpinnings of the CP, informing theoretical models of symbolic knowledge acquisition.

The study plan was refined over four rounds of review, with input from two external reviewers, after which the recommender judged that the Stage 1 manuscript met the criteria for in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/gzpk5

Level of bias control achieved: Level 4. At least some of the data/evidence that will be used to answer the research question already exists and is accessible in principle to the authors (e.g. residing in a public database or with a colleague) but the authors certify that they have not yet accessed any part of that data/evidence.
 
List of eligible PCI RR-friendly Journals:
 
 
References
 
Ivanova, E., Joanisse, M., Ansari, D., & Soltanlou, M. (2024). The origin of symbolic numerical knowledge in early development – an fNIRS Registered Report. In principle acceptance of Version 7 by Peer Community in Registered Reports. https://osf.io/gzpk5
The origin of symbolic numerical knowledge in early development – an fNIRS Registered ReportElizaveta Ivanova, Marc Joanisse, Daniel Ansari, Mojtaba Soltanlou<p>The ability to understand that each number word in the count sequence refers to a specific set of items (e.g., ‘two’ means ‘two things’) is a milestone in cognitive development. When children reach this milestone, they are said to understand th...Social sciencesRobert McIntosh2023-03-14 17:25:27 View
09 Sep 2024
STAGE 1

How Effortful is Boredom? Studying Self-Control Demands Through Pupillometry

Unmasking the effort of boredom: A deeper look into self-control dynamics

Recommended by ORCID_LOGO based on reviews by Jonas Dora, Julia Englert, Erik Bijleveld and 1 anonymous reviewer
Effective effort regulation is central for self-control and has been a focus of psychological research for many decades. It is well established that challenging cognitive tasks, which impose self-control demands due to their complexity, are perceived as more effortful and require greater physiological activation than simpler tasks. However, the experience and allocation of effort during a task, whether easy or difficult, likely depends on more than these inherent self-control demands. Another key factor is boredom, which can arise during task execution. Yet, the role of boredom and the interplay with perceived task difficulty over time remains underexamined.
 
Based on these considerations, Radtke et al. (2024) aim to examine how boredom and task difficulty influence both subjective and objective measures of effort. They propose that subjective cognitive effort consists of both boredom-related and difficulty-related components, hypothesizing that both factors together more accurately predict objective effort allocation than task difficulty alone. The proposed study will use a within-subject design, in which participants perform two versions of the Stroop task: an easy version with only congruent trials and a hard version. Thought probes during the task will track subjective boredom, fatigue, perceived difficulty, and effort. Phasic and stimulus-evoked pupil dilation will serve as an objective index of cognitive effort, while a subsequent flanker task will assess effects on secondary task performance. 
 
The authors predict that the easy Stroop version will result in lower perceived difficulty but higher boredom, as well as fewer errors and faster reaction times compared to the hard version. Over time, boredom and perceived effort due to boredom are expected to increase, while perceived task difficulty and effort attributed to difficulty are anticipated to decrease. Linear Mixed Models will be employed to determine whether phasic and stimulus-evoked pupil dilation can be predicted by effort attributed to boredom, difficulty, or both. This innovative approach combines objective and subjective measures and promises to deepen our understanding of the cognitive demands posed by boredom, with significant implications for research on effort regulation and motivation. 
 
The Stage 1 manuscript was evaluated by four expert reviewers in two rounds of in-depth review. Following responses from the authors, the recommender determined that Stage 1 criteria were met and awarded in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/38qz4

Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly Journals:
 
 
References

Radtke, V., Wolff, W., & Martarelli, C. S. (2024). How effortful is boredom? Studying self-control demands through pupillometry. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/38qz4
How Effortful is Boredom? Studying Self-Control Demands Through PupillometryVanessa C. Radtke, Wanja Wolff, Corinna S. Martarelli<p>Self-control is essential for managing our actions, yet its exertion is perceived as effortful. Performing a task may require effort not only because of its inherent difficulty but also due to its potential for inducing boredom, as boredom has ...Humanities, Social sciencesThomas Meyer2023-11-21 17:56:13 View
09 Sep 2024
STAGE 1

Does concern regarding climate change impact subsequent mental health? A longitudinal analysis using data from the Avon Longitudinal Study of Parents and Children (ALSPAC)

Does concern regarding climate change impact subsequent mental health?

Recommended by ORCID_LOGO based on reviews by Anna Castiglione and Esther Papies
The link between climate change and health is becoming increasingly clear, and problematic (IPCC, 2023). While most agree that climate change is a problem (Vlasceanu et al., 2024) and evidence is starting to emerge that individual's wellbeing can benefit from engaging in proenvironmental behaviour (Prinzing, 2023), there is little robust longitudinal evidence available to make causal claims about the complex and inter-related nature of these types of effects.
 
In their proposed study, Major-Smith et al. (2024) use the Avon Longitudinal Study of Parents and Children to explore whether climate concern has a causal impact upon mental health. Strategic use of this pre-existing data allows consideration of both confounding bias and reverse causality to provide more rigorous evidence for this causal effect. The second research question (whether climate action moderates this relationship) is of both personal and professional interest, as someone who struggles with climate anxiety, and plants hundred of trees annually to try and mitigate the dread. Capable of exploring the potential for individual-level climate action to have a double effect - directly help with climate change mitigation and also an individuals' mental health, the proposed research is a rewarding and insightful line of inquiry in a domain desperate for a more rapid and rigorous evidence-base.
 
The Stage 1 manuscript was evaluated over one round of review. Based on detailed responses to the reviewers' comments, the recommender judged that the manuscript met the Stage 1 criteria and therefore awarded in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/t6d3k
 
Level of bias control achieved: Level 2. At least some data/evidence that will be used to answer the research question has been accessed and partially observed by the authors, but the authors certify that they have not yet observed the key variables within the data that will be used to answer the research question AND they have taken additional steps to maximise bias control and rigour.
 
List of eligible PCI RR-friendly Journals:
 
 
References
 
 
2. Major-Smith, D., Halstead, I., & Major-Smith, K. (2024) Does concern regarding climate change impact subsequent mental health? A longitudinal analysis using data from the Avon Longitudinal Study of Parents and Children (ALSPAC). In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/t6d3k
 
3. Prinzing, M. (2023). Proenvironmental Behavior Increases Subjective Well-Being: Evidence From an Experience-Sampling Study and a Randomized Experiment. Psychological Science. https://doi.org/10.1177/09567976241251766
 
4. Vlasceanu, M., Doell, K. C., Bak-Coleman, J. B., Todorova, B., Berkebile-Weinberg, M. M., Grayson, S. J., ... & Lutz, A. E. (2024). Addressing climate change with behavioral science: A global intervention tournament in 63 countries. Science Advances, 10, eadj5778. https://doi.org/10.1126/sciadv.adj5778
Does concern regarding climate change impact subsequent mental health? A longitudinal analysis using data from the Avon Longitudinal Study of Parents and Children (ALSPAC)Daniel Major-Smith, Isaac Halstead, Katie Major-Smith<p>Climate change is having a substantial – and increasingly severe – impact on our planet, affecting people’s health, security and livelihoods. As a consequence, the concept of ‘climate anxiety’ has recently been developed to characterise the psy...Medical Sciences, Social sciencesThomas Evans2024-05-24 15:50:50 View
06 Sep 2024
STAGE 1

Do individual differences in cognitive ability or personality predict noticing in inattentional blindness tasks?

Are individual difference in inattentional blindness related to cognitive abilities or personality traits?

Recommended by ORCID_LOGO based on reviews by Ruben Arslan and 1 anonymous reviewer
Despite inattentional blindness being a widely recognized and researched phenomenon — popularized by experiments like the invisible gorilla — the evidence on how individual differences affect the occurrence of inattentional blindness has remained inconsistent, largely due to small sample sizes and methodological variations.
 
In this context, Daniel J. Simons, known for his role in bringing public attention to inattentional blindness, along with Yifan Ding, Connor M. Hults, and Brent W. Roberts, presents an ambitious yet well-constructed registered report that addresses this critical gap in the literature. Their report outlines a comprehensive investigation into whether individual differences in cognitive ability or personality traits can predict the likelihood of noticing unexpected objects in various inattentional blindness tasks. The two proposed studies — one focusing on cognitive predictors and the other on personality predictors — arguably represent the most extensive single-sample tests to date on this topic.
 
The authors propose a robust methodology that includes a total of 2,000 participants (1,000 per study). The study design features three distinct inattentional blindness tasks with varying levels of demand to assess the generalizability of the findings across different experimental contexts. In Study 1, cognitive measures such as matrix reasoning and operation span — both well-established indicators of fluid intelligence and working memory — are utilized. Study 2 incorporates a range of personality measures, including the Big Five personality traits and attention-related traits (e.g., ADHD and obsessive-compulsive characteristics).
 
The report also presents a detailed analysis plan with pre-specified primary and secondary outcomes, strong justifications for the sample size, and clearly defined criteria for data inclusion and exclusion. The authors intend to employ multiple statistical techniques, such as correlation analyses and regression models, along with rigorous checks for replicability, to explore the relationship between individual differences and inattentional blindness.
 
Overall, this registered report is a well-justified and meticulously planned investigation into the role of individual differences in inattentional blindness. The proposed studies have the potential to make a significant contribution to our understanding of the cognitive and personality factors that influence the noticing of unexpected objects. The rigorous experimental design, large sample sizes, and adherence to open science practices make this a valuable addition to the literature.

Based on the strengths of the proposal and the authors' responsiveness to the detailed feedback from two reviewers, the recommender justed that the manuscript met the Stage 1 criteria and awarded in-principle acceptance (IPA).

URL to the preregistered Stage 1 protocol: https://osf.io/xtwfs (under temporary private embargo)
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly Journals:
 
 
References
 
Simons, D. J., Ding, Y., Hults, C. M., & Roberts, B. W. (2024). Registered Report: Do individual differences in cognitive ability or personality predict noticing in inattentional blindness tasks? In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/xtwfs
 
Do individual differences in cognitive ability or personality predict noticing in inattentional blindness tasks?Daniel J. Simons, Yifan Ding, Connor M. Hults, Brent W. Roberts<p>People often fail to notice unexpected objects or events when they focus attention on another task or different aspects of a scene. Recently, a number of studies have examined whether individual differences in cognitive abilities or personality...Social sciencesGidon Frischkorn2024-03-28 21:52:33 View
06 Sep 2024
STAGE 1

The Role of Offender Identifiability in Second- and Third-Party Punishment

Punishing Offenders

Recommended by ORCID_LOGO based on reviews by Gilad Feldman and Rajarshi Majumder
When people are less-well-known to us, it can impact how we treat them. People outside our communities may feel more anonymous or de-personalized to us, and this could impact the empathy we feel for them. Indeed, past research shows that people seem to care more for those who are who are identified to us, especially when they are victims of a crime (Small & Loewenstein, 2003). In addition, past research also shows that anonymizing victims can influence criminal behavior (Walters, 2022).  Thus, social cognitive factors related to anonymity appear to affect both positive feelings (engaging in empathy) and negative activities (criminal activity) related to victims. It seems likely that anonymity can also affect how people judge criminals themselves.
 
This paper by Blanke and Twardawski (2024) examines how even minor identifying features can make individuals react differently to a perpetrator of a crime. A picture or name (information that may appear in the press) may impact how people perceive criminals in terms of punishment, empathy, outrage, and blame.
 
How people respond to anonymous or identified criminals may depend on whether the impact of the crime on the victim is made salient. Similar to the Black Sheep Effect (Marques & Paez,1994), the current paper expects that people who identify with victims are expected to punish a perpetrator more when the perpetrator’s name and image are known. This is similar to the case with an ingroup member in The Black Sheep Effect, where ingroup members are punished more for transgressions than outgroup members. For people who view the situation as a third party (and are thus less connected to the victim), an identified perpetrator is expected to lead to less punishment (and also less outrage and blame, and more empathy).
 
The current study focuses on pickpocketing since it is a crime that can be committed without identifying oneself to the victim. Future research should examine how the type of event impacts judgments, especially given the stigma of being a victim of some crimes. Littering and other minor violations may be seen as understandable when you see someone as a person, rather than a anonymous, impersonal criminal.  On the other hand, there can be stigma to being a victim in some cases, such as the case of sexual harassment, where, research shows, perpetrators are judged more positively if they are identified, and victims, especially women, are judged more negatively if they are identified (Barak-Corren & Lewinsohn-Zamir, 2019).
 
People around the world engage in moral and legal transgressions, and if our judgments of them are influenced by minor features such as a photo or name, we should take action to treat people more fairly. This research will help to determine if people are influenced by these factors.
 
The Stage 1 manuscript underwent one round of thorough review. After considering the detailed responses to the reviewers' comments, the recommender determined that the manuscript met the Stage 1 criteria and granted in-principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/7qzva
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly Journals:
 
 
References
 
1. Barak‐Corren, N., & Lewinsohn‐Zamir, D. (2019). What's in a Name? The Disparate Effects of Identifiability on Offenders and Victims of Sexual Harassment. Journal of Empirical Legal Studies, 16, 955-1000. https://doi.org/10.1111/jels.12233
 
2. Blanke, T. & Twardawski, M. (2024). The Role of Offender Identifiability in Second- and Third-Party Punishment. In principle acceptance of Version 2 by Peer Community in Registered Reports. https://osf.io/7qzva
 
3. Marques, J. M., & Paez, D. (1994). The ‘black sheep effect’: Social categorization, rejection of ingroup deviates, and perception of group variability. European Review of Social Psychology, 5, 37-68. https://doi.org/10.1080/14792779543000011
 
4. Small, D. A., & Loewenstein, G. (2003). Helping a victim or helping the victim: Altruism and identifiability. Journal of Risk and Uncertainty, 26, 5-16. https://doi.org/10.1023/A:1022299422219
 
5. Walters, G. D. (2022). Crime and social cognition: A meta-analytic review of the developmental roots of adult criminal thinking. Journal of Experimental Criminology, 18, 183-207. https://doi.org/10.1007/s11292-020-09435-w
The Role of Offender Identifiability in Second- and Third-Party PunishmentTheresa Blanke, Mathias Twardawski<p>The identifiability effect describes humans' tendency to exhibit different emotional and behavioral responses toward identified as compared to anonymous individuals. This phenomenon has been extensively studied within the identifiable victim ef...Social sciencesClare Conry-Murray Rajarshi Majumder, Gilad Feldman2024-03-23 11:02:30 View
06 Sep 2024
STAGE 2
(Go to stage 1)

One and only SNARC? Spatial-Numerical Associations are not fully flexible and depend on both relative and absolute magnitude

A Registered Report demonstration that the SNARC effect depends on absolute as well as relative number magnitude

Recommended by ORCID_LOGO based on reviews by Claudia Gianelli
The Spatial-Numerical Association of Response Codes (SNARC) effect refers to the fact that smaller numbers receive faster responses with the left hand, and larger numbers with the right hand (Dehaene et al., 1993). This robust finding implies that numbers are associated with space, being represented on a mental number line that progresses from left to right. The SNARC effect is held to depend on relative number magnitude, with the mental number line dynamically adjusting to the numerical range used in a given context. This characterisation is based on significant effects of relative number magnitude, with no significant influence of absolute number magnitude. However, a failure to reject the null hypothesis is not firm evidence for the absence of an effect. In this Registered Report, Roth and colleagues (2024) report two large-sample online experiments, with a Bayesian statistical approach to confirm—or refute—a role for absolute number magnitude in modulating the classic SNARC effect (smallest effect size of interest, d = 0.15).
 
Experiment 1 closely followed Dehaene’s (1993) original methods, and found strong evidence for an influence of relative magnitude, and moderate-to-strong evidence against an influence of absolute magnitude. Experiment 2 was designed to exclude some potential confounds in the original method, and this second experiment found strong evidence for both relative and absolute magnitude effects, of comparable effect sizes (in the range of d = .24 to .42). This registered study demonstrates that the SNARC effect is not ‘fully flexible’, in the sense of depending only on relative number magnitude; it is also shaped by absolute magnitude.
 
This Stage 2 manuscript was evaluated by the recommender and one external reviewer. Following appropriate minor revisions, the recommender judged that the manuscript met the Stage 2 criteria for recommendation.
 
URL to the preregistered Stage 1 protocol: https://osf.io/ae2c8
 
Level of bias control achieved: Level 6. No part of the data or evidence that was used to answer the research question was generated until after IPA.
 
List of eligible PCI RR-friendly journals:
 
 
References
 
1. Dehaene, S., Bossini, S., & Giraux, P. (1993). The mental representation of parity and number magnitude. Journal of Experimental Psychology: General, 122, 371–396. https://doi.org/10.1037/0096-3445.122.3.371
 
2. Roth, L., Caffier, J., Reips, U.-D., Nuerk, H.-C., Overlander, A. T. & Cipora, K. (2023). One and only SNARC? Spatial-Numerical Associations are not fully flexible and depend on both relative and absolute magnitude [Stage 2]. Acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/epnd4
One and only SNARC? Spatial-Numerical Associations are not fully flexible and depend on both relative and absolute magnitudeLilly Roth, John Caffier, Ulf-Dietrich Reips, Hans-Christoph Nuerk, Annika Tave Overlander, Krzysztof Cipora<p>Numbers are associated with space, but it is unclear how flexible these associations are. We investigated whether the SNARC effect (Spatial-Numerical Association of Response Codes; Dehaene et al., 1993; i.e., faster responses to small/large num...Life SciencesRobert McIntosh2024-06-10 15:00:30 View
06 Sep 2024
STAGE 1

Barriers and facilitators to the adoption and promotion of Open Science practices in psychology. The case of Slovakia

What are the barriers and facilitators to open science practices for researchers, policy makers and media representatives in Slovakia?

Recommended by ORCID_LOGO based on reviews by Crystal Steltenpohl, Peter Branney, Andrea E. Abele-Brehm , Emma Norris and 1 anonymous reviewer
Open science practices (OSPs, e.g., preregistration, open materials, code and data) aim to enhance the transparency, integrity, and reproducibility of research. Recent work, however, has highlighted various facilitators and barriers perceived by researchers in implementing these, which can either enhance or hinder their success. Little is known about these barriers in the context of Slovakia, and such perceptions are rarely investigated for policy makers and media representatives who are also embedded in the research ecosystem.
 
In their Stage 1 Registered Report, Marcel Martončik and colleagues aim to map the perceptions and experiences of barriers and facilitators of OSPs that are unique to different stakeholder groups in Slovakia. They will conduct both semi-structured interviews and focus groups with a diverse sample of postgraduate students, researchers, policymakers, and media representatives from the field of psychology. Reflexive thematic analysis will identify overarching themes regarding such barriers and facilitators which will provide valuable insights into the support required to make OSPs normative across different stakeholder groups.
 
Four expert reviewers assessed the Stage 1 manuscript across two rounds of in-depth review. Based on the authors' detailed and informed responses to the reviewer’s comments, the recommender judged that the manuscript met the Stage 1 criteria and awarded in principle acceptance (IPA).
 
URL to the preregistered Stage 1 protocol: https://osf.io/n86um
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly Journals:
 
 
References
 
Martončik, M., Adamkovič, M., Baník G., Fedáková, D., Issmailová, S., Kačmár, P., Kentoš, M., Majdáková, V., Papcunová, J., & Vargová, L. (2024). Barriers and facilitators to the adoption and promotion of Open Science practices in psychology. The case of Slovakia. In principle acceptance of Version 1.1 by Peer Community in Registered Reports. https://osf.io/n86um
Barriers and facilitators to the adoption and promotion of Open Science practices in psychology. The case of SlovakiaMarcel Martončik, Matúš Adamkovič, Gabriel Baník, Denisa Fedáková, Samar Issmailová, Pavol Kačmár, Michal Kentoš, Viktória Majdáková, Jana Papcunová, Lenka Vargová<p>Various responsible research practices emphasizing transparency, such as open<br>data, open code, open peer review, and preregistration, have been introduced to<br>enhance the reproducibility and replicability of findings. The ongoing initiativ...Social sciencesCharlotte Pennington2024-04-29 14:39:12 View
22 Jul 2024
STAGE 1
article picture

Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”

Replicating, Revising and Reforming: Unpicking the Apparent Nanoparticle Endosomal Escape Paradox

Recommended by and ORCID_LOGO based on reviews by Cecilia Menard-Moyon and Zeljka Krpetic
Context
 
Over the past decade there has been an exponential increase in the number of research papers utlising nanoparticles for biological applications such as intracellular sensing [1, 2], theranostics [3-5] and more recently drug delivery and precision medicine [6, 7]. Despite the success stories, there is a disconnect regarding current dogma on issues such as nanoparticle uptake and trafficking, nanoparticle delivery via the enhanced permeability and retention (EPR) effect, and endosomal escape. Critical re-evaluation of these concepts both conceptually and experimentally is needed for continued advancement in the field.
 
For this preregistration, Said et al. (2024) focus on nanoparticle intracellular trafficking, specifically endosomal escape [8]. The current consensus in the literature is that nanoparticles enter cells via endocytosis [9, 10] but reportedly just 1-2% of nanoparticles/ nanoparticle probes escape endosomes and enter the cytoplasm [11-13]. There is therefore an apparent paradox over how sensing nanoparticles can detect their intended targets in the cytoplasm if they are trapped within the cell endosomes. To address this fundamental issue of nanoparticle endosomal escape, Lévy and coworkers are carrying out replication studies to thoroughly and transparently replicate the most influential papers in the field of nanoparticle sensing. The aim of these replication studies is twofold: to establish a robust methodology to study endosomal escape of nanoparticles, and to encourage discussions, transparency and a step-change in the field.  
 
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”
 
For this replication study, the authors classified papers on the topic of nanoparticle sensing and subsequently ranked them by number of citations.  Based on this evaluation they selected a paper by Zhu and colleagues [14] entitled “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions” for their seminal replication study.  To determine the reproducibility of the results from Zhu et al., the authors aim to establish the proportion of endosomal escape of the nanoparticles, and to examine the data in a biological context relevant to the application of the probe.
 
Beyond Replication
 
The authors plan to replicate the exact conditions reported in the materials and methods section of the selected paper such as nanoparticle probe synthesis of CdSe@C-TPEA nanoparticles, assessment of particle size, stability and reactivity and effect on cells (TEM, pH experiments, fluorescent responsivity to metal ions and cell viability). In addition, Said et al., plan to include further experimental characterisation to complement the existing study by Zhu and colleagues. They will incorporate additional controls and methodology to determine the intracellular location of nanoparticle probes in cells including: quantifying excess fluorescence in the culture medium, live cell imaging analysis, immunofluorescence with endosomal and lysosomal markers, and electron microscopy of cell sections. The authors will also include supplementary viability studies to assess the impact of the nanoparticles on HeLa cells as well as an additional biologically relevant cell line (for use in conjunction with the HeLa cells as per the original paper).
 
The Stage 1 manuscript underwent two rounds of thorough in-depth review. After considering the detailed responses to the reviewers' comments, the recommenders  determined that the manuscript met the Stage 1 criteria and awarded in-principle acceptance (IPA).  
 
The authors have thoughtfully considered their experimental approach to the replication study, whilst acknowledging any potential limitations. Given that conducting such a replication study is novel in the field of Nanotechnology and there is currently no ‘gold standard’ approach in doing so, the authors have showed thoughtful regard of statistical analysis and unbiased methodology where possible.
 
Based on current information, this study is the first use of preregistration via Peer Community in Registered Reports and the first formalised replication study in Nanotechnology for Biosciences. The outcomes of this of this study will be significant both scientifically and in the wider context in discussion of the scientific method.
 
URL to the preregistered Stage 1 protocol: https://osf.io/qbxpf
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly Journals:
 
References
 
1. Howes, P. D., Chandrawati, R., & Stevens, M. M. (2014). Colloidal nanoparticles as advanced biological sensors. Science, 346(6205), 1247390. https://doi.org/10.1126/science.1247390
 
2. Liu, C. G., Han, Y. H., Kankala, R. K., Wang, S. B., & Chen, A. Z. (2020). Subcellular performance of nanoparticles in cancer therapy. International Journal of Nanomedicine, 675-704. https://doi.org/10.2147/IJN.S226186
 
3. Tang, W., Fan, W., Lau, J., Deng, L., Shen, Z., & Chen, X. (2019). Emerging blood–brain-barrier-crossing nanotechnology for brain cancer theranostics. Chemical Society Reviews, 48(11), 2967-3014. https://doi.org/10.1039/C8CS00805A
 
4. Yoon, Y. I., Pang, X., Jung, S., Zhang, G., Kong, M., Liu, G., & Chen, X. (2018). Smart gold nanoparticle-stabilized ultrasound microbubbles as cancer theranostics. Journal of Materials Chemistry B, 6(20), 3235-3239. https://doi.org/10.1039%2FC8TB00368H
 
5. Lin, H., Chen, Y., & Shi, J. (2018). Nanoparticle-triggered in situ catalytic chemical reactions for tumour-specific therapy. Chemical Society Reviews, 47(6), 1938-1958. https://doi.org/10.1039/C7CS00471K
 
6. Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021). Lipid nanoparticles for mRNA delivery. Nature Reviews Materials, 6(12), 1078-1094. https://doi.org/10.1038/s41578-021-00358-0
 
7. Mitchell, M. J., Billingsley, M. M., Haley, R. M., Wechsler, M. E., Peppas, N. A., & Langer, R. (2021). Engineering precision nanoparticles for drug delivery. Nature Reviews Drug Discovery, 20(2), 101-124. https://doi.org/10.1038/s41573-020-0090-8
 
8. Said, M., Gharib, M., Zrig, S., & Lévy, R. (2024). Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”. In principle acceptance of Version 3 by Peer Community in Registered Reports. https://osf.io/qbxpf
 
9. Behzadi, S., Serpooshan, V., Tao, W., Hamaly, M. A., Alkawareek, M. Y., Dreaden, E. C., ... & Mahmoudi, M. (2017). Cellular uptake of nanoparticles: Journey inside the cell. Chemical Society Reviews, 46(14), 4218-4244. https://doi.org/10.1039/C6CS00636A
 
10. de Almeida, M. S., Susnik, E., Drasler, B., Taladriz-Blanco, P., Petri-Fink, A., & Rothen-Rutishauser, B. (2021). Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine. Chemical society reviews, 50(9), 5397-5434. https://doi.org/10.1039/D0CS01127D
 
11. Smith, S. A., Selby, L. I., Johnston, A. P., & Such, G. K. (2018). The endosomal escape of nanoparticles: toward more efficient cellular delivery. Bioconjugate Chemistry, 30(2), 263-272. http://dx.doi.org/10.1021/acs.bioconjchem.8b00732
 
12. Cupic, K. I., Rennick, J. J., Johnston, A. P., & Such, G. K. (2019). Controlling endosomal escape using nanoparticle composition: current progress and future perspectives. Nanomedicine, 14(2), 215-223. https://doi.org/10.2217/nnm-2018-0326
 
13. Wang, Y., & Huang, L. (2013). A window onto siRNA delivery. Nature Biotechnology, 31(7), 611-612. https://doi.org/10.1038/nbt.2634
 
14. Zhu, A., Qu, Q., Shao, X., Kong, B., & Tian, Y. (2012). Carbon-dot-based dual-emission nanohybrid produces a ratiometric fluorescent sensor for in vivo imaging of cellular copper ions. Angewandte Chemie (International ed. in English), 51(29), 7185-7189. https://doi.org/10.1002/anie.201109089
Replication of “Carbon-Dot-Based Dual-Emission Nanohybrid Produces a Ratiometric Fluorescent Sensor for In Vivo Imaging of Cellular Copper Ions”Maha Said, Mustafa Gharib, Samia Zrig, Raphaël Lévy <p>In hundreds of articles published over the past two decades, nanoparticles have been described as probes for sensing and imaging of a variety of intracellular cytosolic targets. However, nanoparticles generally enter cells by endocytosis with o...Life Sciences, Medical Sciences, Physical SciencesEmily Linnane2023-11-29 19:14:03 View
22 Jul 2024
STAGE 1

Probing the dual-task structure of a metacontrast-masked priming paradigm with subjective visibility judgments

Do trial-wise visibility reports - and how these reports are made - alter unconscious priming effects?

Recommended by ORCID_LOGO based on reviews by Markus Kiefer, Thomas Schmidt and 3 anonymous reviewers
Many studies of unconscious processing measure priming effects. Such experiments test whether a prime stimulus can exert an effect on speeded responses to a subsequently presented target stimulus even when participants are unaware of the prime. In some studies, participants are required to report their awareness of the prime in each trial - a dual-task design. Other studies conduct such visibility tests in separate experiments, so that the priming effect is measured via a single task. Both these approaches have pros and cons; however, it remains unclear to what extent they can affect the process of interest. Can the choice of experimental design and its parameters interfere with the priming effect? This could have implications for interpreting such effects, including in previous literature.
 
In the current study, Wendt and Hesselmann (2024) will investigate the effects of using a dual-task design in a masked priming paradigm, focusing on subjective visibility judgments. Based on power analysis, the study will test 34 participants performing both single-task and several dual-task conditions to measure reaction times and priming effects. Priming is tested via a speeded forced-choice identification of a target. The key manipulation is the non-speeded visibility rating of the prime using a Perceptual Awareness Scale, either with a graded (complex) rating or a dichotomous response. Moreover, participants will either provide their awareness judgement via a keyboard or vocally. Finally, participants will also complete a control condition to test prime visibility by testing the objective identification of the prime. These conditions will be presented in separate blocks, with the order randomised across participants. The authors hypothesise that using a dual-task slows down response times and boosts priming effects. However, they further posit that keyboard responses and graded visibility ratings, respectively, in the dual task reduce priming effects (but also slow response times) compared to vocal responses and dichotomous visibility judgements. In addition to the preregistered hypotheses, the study will also collect EEG data to explore the neural underpinnings of these processes.
 
The Stage 1 manuscript went through three rounds of review by the recommender and five expert reviewers. While the recommender would have preferred to see targeted, directional hypotheses explicitly specified in the design instead of non-directional main effects/interactions, he nevertheless considers this experimental design ready for commencing data collection, and therefore granted in-principle acceptance.
 
URL to the preregistered Stage 1 protocol: https://osf.io/ds2w5
 
Level of bias control achieved: Level 6. No part of the data or evidence that will be used to answer the research question yet exists and no part will be generated until after IPA.
 
List of eligible PCI RR-friendly Journals:
 

References

Wendt, C. & Hesselmann, G. (2024). Probing the dual-task structure of a metacontrast-masked priming paradigm with subjective visibility judgments. In principle acceptance of Version 4 by Peer Community in Registered Reports. https://osf.io/ds2w5
Probing the dual-task structure of a metacontrast-masked priming paradigm with subjective visibility judgmentsCharlott Wendt, Guido Hesselmann<p>Experiments contrasting conscious and masked stimulus processing have shaped, and continue to shape, cognitive and neurobiological theories of consciousness. However, as shown by Aru et al. (2012) the contrastive approach builds on the untenabl...Social sciencesD. Samuel Schwarzkopf2024-03-02 18:20:03 View